VWF ELISA Kit

Catalog No. ABIN2703573

Product Details VWF ELISA Kit

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.13-120 ng/mL

Minimum Detection Limit: 0.13 ng/mL

Application: ELISA

Purpose: Human VWF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human vWF. Other species not determined.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for VWF ELISA Kit (ABIN2703573)

Małkiewicz, Słomiński, Skrzypkowska, Siebert, Gutknecht, Myśliwska: "The GA genotype of the -1154 G/A (rs1570360) vascular endothelial growth factor (VEGF) is protective against hypertension-related chronic kidney disease incidence." in: Molecular and cellular biochemistry, Vol. 418, Issue 1-2, pp. 159-65, (2016) (PubMed).

Małkiewicz, Myśliwska, Skrzypkowska, Słomiński, Siebert, Gutknecht: "Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease." in: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 38, Issue 5, pp. 469-75, (2016) (PubMed).

Małkiewicz, Skrzypkowska, Słomiński, Siebert, Gutknecht, Myśliwska: "The GG genotype of the -152 G/A vascular endothelial growth factor (VEGF) polymorphism predisposes to hypertension-related chronic kidney disease." in: Molecular biology reports, Vol. 43, Issue 9, pp. 967-75, (2016) (PubMed).

Ma, Su, Zhang, Ling, Yin, Bai, Ruan: "The co-influence of VWD type 2B/2M mutations in the A1 domain and platelet GPIbα on the rate of cleavage to VWF by ADAMTS13." in: Thrombosis research, Vol. 136, Issue 5, pp. 987-95, (2015) (PubMed).

Zhai, Chen, Gao, Ma, Chen, Yu, Li, Liu, Xiang, Xie, Yang: "Inhibition of the NF-κB pathway by R65 ribozyme gene via adeno-associated virus serotype 9 ameliorated oxidized LDL induced human umbilical vein endothelial cell injury." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9912-21, (2015) (PubMed).

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: vWF (VWF Products)

Synonyms: VWF ELISA Kit, si:ch1073-474e24.1 ELISA Kit, F8VWF ELISA Kit, VWD ELISA Kit, 6820430P06Rik ELISA Kit, AI551257 ELISA Kit, B130011O06Rik ELISA Kit, C630030D09 ELISA Kit, von Willebrand factor ELISA Kit, Von Willebrand factor ELISA Kit, VWF ELISA Kit, vwf ELISA Kit, Vwf ELISA Kit

Background: Gene Names: VWF F8VWF
Protein names: von Willebrand factor (vWF) [Cleaved into: von Willebrand antigen 2 (von Willebrand antigen II)]

Gene ID: 7450

UniProt: P04275