ACVR2A ELISA Kit
-
- Target See all ACVR2A ELISA Kits
- ACVR2A (Activin A Receptor, Type IIA (ACVR2A))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Activin A Receptor Type II A (ACVR2A). No significant cross-reactivity or interference between Activin A Receptor Type II A (ACVR2A) and analogues was observed.
- Sensitivity
- 0.055 ng/mL
- Featured
- Discover our best selling ACVR2A ELISA Kit
- Top Product
- Discover our top product ACVR2A ELISA Kit
-
-
- Comment
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type II A (ACVR2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type II A (ACVR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type II A (ACVR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type II A (ACVR2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A Receptor Type II A (ACVR2A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A Receptor Type II A (ACVR2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
-
- Target See all ACVR2A ELISA Kits
- ACVR2A (Activin A Receptor, Type IIA (ACVR2A))
- Alternative Name
- Activin A Receptor Type II A (ACVR2A Products)
- Synonyms
- ACVR2 ELISA Kit, ACVR2A ELISA Kit, XSTK9 ELISA Kit, actrii ELISA Kit, acvr2 ELISA Kit, ACTRII ELISA Kit, Acvr2 ELISA Kit, rActR-II ELISA Kit, actr2a ELISA Kit, acvr2a ELISA Kit, wu:fa02e10 ELISA Kit, ActrIIa ELISA Kit, TactrII ELISA Kit, activin A receptor type 2A ELISA Kit, activin A receptor type 2A L homeolog ELISA Kit, activin A receptor type 2Aa ELISA Kit, activin receptor IIA ELISA Kit, ACVR2A ELISA Kit, acvr2a ELISA Kit, Acvr2a ELISA Kit, acvr2a.L ELISA Kit, acvr2aa ELISA Kit
- Background
-
Gene Name: Activin A Receptor Type II A
Gene Aliases: ACVR2-A, ACTRII, ACTR-II, ACVR2
- Gene ID
- 92
- UniProt
- P27037
-