GGT1 ELISA Kit

Catalog No. ABIN5654506

Product Details GGT1 ELISA Kit

Target: GGT1 (gamma Glutamyltransferase 1 (GGT1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Gamma-Glutamyltransferase 1 (gGT1). No significant cross-reactivity or interference between Gamma-Glutamyltransferase 1 (gGT1) and analogues was observed.

Sensitivity: 0.61 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyltransferase 1 (gGT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyltransferase 1 (gGT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyltransferase 1 (gGT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Glutamyltransferase 1 (gGT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyltransferase 1 (gGT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyltransferase 1 (gGT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GGT1

Target: GGT1 (gamma Glutamyltransferase 1 (GGT1))

Alternative Name: Gamma-Glutamyltransferase 1 (GGT1 Products)

Synonyms: ggt1 ELISA Kit, zgc:64172 ELISA Kit, CD224 ELISA Kit, D22S672 ELISA Kit, D22S732 ELISA Kit, GGT ELISA Kit, GGT 1 ELISA Kit, GTG ELISA Kit, GGT-1 ELISA Kit, Ggtp ELISA Kit, dwg ELISA Kit, GGLUT ELISA Kit, Ggt ELISA Kit, GGT2 ELISA Kit, cd224 ELISA Kit, ggt ELISA Kit, gtg ELISA Kit, GGT1 ELISA Kit, ggt1-a ELISA Kit, gamma-glutamyltransferase 1a ELISA Kit, gamma-glutamyltransferase 1 ELISA Kit, gamma-glutamyltranspeptidase 1 ELISA Kit, gamma-glutamyltransferase 1 L homeolog ELISA Kit, gamma-glutamyltransferase ELISA Kit, gamma-glutamyltranspeptidase Ggt1 ELISA Kit, ggt1a ELISA Kit, GGT1 ELISA Kit, Ggt1 ELISA Kit, ggt1 ELISA Kit, LOC470168 ELISA Kit, ggt1.L ELISA Kit, ggt ELISA Kit, Bcep18194_A6223 ELISA Kit, h16_A1098 ELISA Kit, Shewana3_2487 ELISA Kit, Bcep1808_2978 ELISA Kit

Background:

Gene Name: Gamma-Glutamyltransferase 1

Gene Aliases: CD224, G-GT1, GTG, Gamma-glutamyltranspeptidase 1, Glutathione hydrolase 1, Leukotriene-C4 hydrolase,

Gene ID: 14598

UniProt: Q60928