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HTRA2 ELISA Kit

HTRA2 Reactivity: Human Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625463
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    HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
    Reactivity
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.2-50 ng/mL
    Minimum Detection Limit
    0.2 ng/mL
    Application
    ELISA
    Purpose
    Human HTRA2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detect human HtrA2. Other species not determined yet.
    Sensitivity
    200 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all HTRA2 ELISA Kits
    HTRA2 (HtrA Serine Peptidase 2 (HTRA2))
    Alternative Name
    HTRA2 (HTRA2 Products)
    Synonyms
    CG8464 ELISA Kit, DmHtrA2 ELISA Kit, Dmel\\CG8464 ELISA Kit, HtrA2/Omi ELISA Kit, Omi ELISA Kit, dOmi ELISA Kit, domi ELISA Kit, htrA2 ELISA Kit, omi ELISA Kit, HTRA2 ELISA Kit, PRSS25 ELISA Kit, OMI ELISA Kit, PARK13 ELISA Kit, AI481710 ELISA Kit, Prss25 ELISA Kit, mnd2 ELISA Kit, HTRA2-related serine protease ELISA Kit, HtrA serine peptidase 2 ELISA Kit, HtrA2 ELISA Kit, HTRA2 ELISA Kit, htra2 ELISA Kit, Htra2 ELISA Kit
    Background
    Gene Names: HTRA2 OMI PRSS25
    Protein names: Serine protease HTRA2, mitochondrial (EC 3.4.21.108) (High temperature requirement protein A2) (HtrA2) (Omi stress-regulated endoprotease) (Serine protease 25) (Serine proteinase OMI)
    Gene ID
    27429
    UniProt
    O43464
    Pathways
    Positive Regulation of Endopeptidase Activity
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