anti-Dog (Canine) BCL2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • Bcl-2
  • PPP1R50
  • bcl-2
  • AW986256
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • BCL-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2
  • B-cell leukemia/lymphoma 2-like
  • B-cell CLL/lymphoma 2 protein
  • BCL2
  • Bcl2
  • LOC100224888
  • bcl2
  • LOC100359045
  • BCL-2
Epitope
AA 41-54
163
78
67
65
44
31
25
25
24
21
18
15
15
15
13
13
13
11
9
9
6
6
6
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Monkey, Pig (Porcine)
1060
258
213
53
27
22
18
17
5
4
4
3
2
2
2
2
Host
Mouse
578
547
19
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
50
50
45
38
25
24
22
16
15
14
14
14
14
14
14
14
13
13
13
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
838
397
387
341
335
327
235
195
171
81
40
15
14
13
12
11
9
4
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN337080
$ 595.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4283360 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 6
1 ABIN4283363 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN957155 IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal
1 ABIN4283369 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN386113 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN957156 IHC (fro) IHC (p) IP WB Rabbit AA 41-54 Log in to see Polyclonal
1 ABIN386116 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN386117 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN386114 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN2738524 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 41-54 Log in to see 124
1 ABIN2738525 FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 41-54 Log in to see 100-D5-124
1 ABIN2738523 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8
1 ABIN2738522 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa Log in to see 100-D5
1 ABIN4283362 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4283359 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN4283368 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(163), (78), (67), (65), (44), (31), (25), (25), (24), (21), (18), (15), (15), (15), (13), (13), (13), (11), (9), (9), (6), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Monkey, Pig (Porcine)
(1060), (258), (213), (53), (27), (22), (18), (17), (5), (4), (4), (3), (2), (2), (2), (2)
Host Mouse
(578), (547), (19), (6), (2), (2), (2)
Clonality
Monoclonal
Conjugate This BCL2 antibody is un-conjugated
(50), (50), (45), (38), (25), (24), (22), (16), (15), (14), (14), (14), (14), (14), (14), (14), (13), (13), (13), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(838), (397), (387), (341), (335), (327), (235), (195), (171), (81), (40), (15), (14), (13), (12), (11), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human Bcl-2a at ~25kD. Species cross-reactivity: monkey. Does not react with mouse or rat.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%) Bovine (93%) Horse (86%).
Purification Protein G purified
Immunogen Synthetic peptide corresponding to residues 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein. Cellular Localization: Cytoplasm. MW of antigen: 25-26kD. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%), Bovine (93%), Horse (86%).

Type of Immunogen: Synthetic peptide
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BCL2 / Bcl-2 (BCL2 Antibody Abstract)
Background Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Approved: ELISA, Flo, IF, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL for 2hrs at RT. Positive control: Tonsil or follicular lymphomas. Raji or HeLa cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4.
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment +4°C or -20°C, Avoid repeated freezing and thawing.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (ABIN337080) Human Spleen (formalin-fixed, paraffin-embedded) stained with BCL2 antibody ABIN33708...