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anti-Human BCL2 antibody for Enzyme Immunoassay

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
BH3 Domain
165
72
64
55
50
44
23
23
22
19
18
15
14
14
13
13
13
13
12
11
7
5
4
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
964
226
164
39
22
16
14
7
4
3
2
2
1
1
Host
Rabbit
521
510
21
6
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
46
46
40
37
24
23
19
14
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
6
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
748
383
337
312
309
302
217
198
168
67
42
12
11
11
10
9
5
3
1
1
1
1
1
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Catalog No. ABIN356812
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN782948 EIA WB Goat N-Term Log in to see Polyclonal 2
1 ABIN374455 EIA WB Goat N-Term Log in to see Polyclonal 2
1 ABIN499427 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN499429 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 1
1 ABIN950625 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
1 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
1 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012
1 ABIN2716564 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Enzyme Immunoassay

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 12 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 352 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 284 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 184 Antibodies
Immunofluorescence (IF) 296 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 301 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 165 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 330 Antibodies
Immunoprecipitation (IP) 203 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope BH3 Domain
(165), (72), (64), (55), (50), (44), (23), (23), (22), (19), (18), (15), (14), (14), (13), (13), (13), (13), (12), (11), (7), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(964), (226), (164), (39), (22), (16), (14), (7), (4), (3), (2), (2), (1), (1)
Host Rabbit
(521), (510), (21), (6), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(46), (46), (40), (37), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (6), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(748), (383), (337), (312), (309), (302), (217), (198), (168), (67), (42), (12), (11), (11), (10), (9), (5), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images
Specificity This antibody is specific to Bcl-2 (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Isotype Ig

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596, 9606
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunoprecipitation: 1/100-1/500Flow Cytometry: 1/100-1/500. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN356812) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BCL2 Antibody (ABIN356812)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) back to top
Supplier Images
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (Image 2)