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anti-Human BCL2 antibody for Enzyme Immunoassay

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
BH3 Domain
169
71
63
54
50
44
23
23
23
19
17
15
14
14
13
13
13
13
12
11
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
954
221
167
40
23
15
7
4
3
3
2
2
2
1
Host
Rabbit
523
507
19
5
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
766
341
321
301
291
281
256
180
154
67
42
11
10
9
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN356812
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN782948 EIA WB Goat N-Term Log in to see Polyclonal 2
0.0008867729 ABIN374455 EIA WB Goat N-Term Log in to see Polyclonal 2
0.0008867729 ABIN499427 EIA IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 1
0.0008867729 ABIN499429 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 1
0.0008867729 ABIN950625 EIA IF IHC (p) WB Rabbit IgG AA 145-157, Internal Region Log in to see Polyclonal
0.0008867729 ABIN567617 EIA IF WB Mouse IgG2b Log in to see AT1B5
0.0008867729 ABIN1105505 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 8-00E-012
0.0008867729 ABIN2716564 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-BCL2 antibody for Enzyme Immunoassay

Similar anti-BCL2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies
ELISA 312 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 279 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 168 Antibodies
Immunofluorescence (IF) 278 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 277 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 152 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 318 Antibodies
Immunoprecipitation (IP) 235 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope BH3 Domain
(169), (71), (63), (54), (50), (44), (23), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (11), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(954), (221), (167), (40), (23), (15), (7), (4), (3), (3), (2), (2), (2), (1)
Host Rabbit
(523), (507), (19), (5), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(766), (341), (321), (301), (291), (281), (256), (180), (154), (67), (42), (11), (10), (9), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images
Specificity This antibody is specific to Bcl-2 (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Isotype Ig

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Synonyms: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596, 9606
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN356812) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunoprecipitation: 1/100-1/500Flow Cytometry: 1/100-1/500. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN356812) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BCL2 Antibody (ABIN356812)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Background publications

Liu, Zhou, Li, Sun, Lin, Lang, He, Cao, Yan, Wang, Lu, Han, Cao, Zhang, Zeng: "Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues." in: Cancer research, Vol. 68, Issue 21, pp. 8871-80, 2008

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN356812) back to top
Supplier Images
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody
 image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (BH3 Domain) (ABIN356812) anti-B-Cell CLL/lymphoma 2 (BCL2) (BH3 Domain) antibody (Image 2)