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anti-Human BCL2 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
AA 41-54
164
72
60
55
50
44
24
24
22
19
17
15
14
14
14
14
14
13
12
12
7
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
958
225
167
40
22
16
15
7
4
3
2
2
1
1
Host
Mouse
519
509
19
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
46
46
40
37
24
23
19
14
14
13
13
13
13
13
13
13
12
12
12
12
6
6
6
6
6
6
6
2
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
760
340
318
292
290
280
252
176
145
74
42
12
10
10
10
5
3
1
1
1
1
1
Supplier
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Catalog No. ABIN967321
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000870507 ABIN967322 IHC (f) IHC (fro) FACS WB Mouse IgG1 AA 41-54 Log in to see 7
0.000870507 ABIN1177235 IHC (f) ICS WB Mouse IgG1 pSer70 Log in to see N46-467 5
0.000870507 ABIN638444 FACS ICC IHC (f) IHC (p) IP WB PE Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 5
0.000870507 ABIN1981878 IHC (f) ICC FACS IP IHC (p) WB APC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 3
0.000870507 ABIN1019888 IHC (f) IHC (p) Rabbit IgG Log in to see E17
0.000870507 ABIN1019889 IHC (f) IHC (p) Rabbit IgG Log in to see E17
0.000870507 ABIN1019891 IHC (f) IHC (p) Mouse IgG1 kappa Log in to see A57H
0.000870507 ABIN1019890 IHC (f) IHC (p) Mouse IgG1 kappa Log in to see A57H
0.000870507 ABIN1302482 FACS ICC IHC (f) IHC (p) IP WB Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4
0.000870507 ABIN1302954 FACS ICC IHC (f) IHC (p) IP WB FITC Mouse IgG1 AA 41-54 Log in to see Bcl-2-100 4

Top referenced anti-BCL2 antibody for Immunohistochemistry (Formalin-fixed Sections)

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Application / Reactivity Human
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Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 275 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 162 Antibodies
Immunofluorescence (IF) 274 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 62 Antibodies
Immunohistochemistry (IHC) 272 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 143 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 310 Antibodies
Immunoprecipitation (IP) 228 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(164), (72), (60), (55), (50), (44), (24), (24), (22), (19), (17), (15), (14), (14), (14), (14), (14), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(958), (225), (167), (40), (22), (16), (15), (7), (4), (3), (2), (2), (1), (1)
Host Mouse
(519), (509), (19), (6), (2), (2), (2)
Clonality
Monoclonal
Conjugate This BCL2 antibody is un-conjugated
(46), (46), (40), (37), (24), (23), (19), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (6), (6), (6), (6), (6), (6), (6), (2), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
(760), (340), (318), (292), (290), (280), (252), (176), (145), (74), (42), (12), (10), (10), (10), (5), (3), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN967321) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Bcl-2 synthetic peptide aa. 41-54
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN967321) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival, rather than by accelerating the rate of cell proliferation. Human Bcl-2 protein migrates at a molecular weight of ~26 kDa by SDS-PAGE. Bcl-2/100 recognizes a 26 kDa band representing human Bcl-2. Additional minor bands at 27-31 kDa and 18-21 kDa may also be visualized. The 27-31 kDa upper band may represent a larger isoform, whereas the 18-21 kDa lower band may be an internal translation or proteolytic product, therefore, a synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Bcl-2/100 does not cross-react with mouse Bcl-2. For detection of mouse Bcl-2 refer to clone 3F11 (ABIN967441), polyclonal rabbit anti-rat/mouse Bcl-2 antiserum, and polyclonal rabbit anti-mouse Bcl-2 antiserum.
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN967321) Images back to top
Application Notes Western Blot: Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Store lysate at -20° C). Additional control lysate (ABIN968537) is sold separately.
IHC: For paraffin-embedded sections, tissues should be fixed in either Bouin's fixative or formalin. For immunofluorescent staining and flow cytometry, our directly conjugated formats are recommended.
Comment

Related Products: ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN967321) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-BCL2 Antibody (ABIN967321)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Krajewski, Tanaka, Takayama, Schibler, Fenton, Reed: "Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes." in: Cancer research, Vol. 53, Issue 19, pp. 4701-14, 1993

Pezzella, Turley, Kuzu, Tungekar, Dunnill, Pierce, Harris, Gatter, Mason: "bcl-2 protein in non-small-cell lung carcinoma." in: The New England journal of medicine, Vol. 329, Issue 10, pp. 690-4, 1993

Tanaka, Saito, Reed: "Structure-function analysis of the Bcl-2 oncoprotein. Addition of a heterologous transmembrane domain to portions of the Bcl-2 beta protein restores function as a regulator of cell survival." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10920-6, 1993

Pezzella, Jones, Ralfkiaer, Ersbøll, Gatter, Mason: "Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma." in: British journal of cancer, Vol. 65, Issue 1, pp. 87-9, 1992

Reed, Meister, Tanaka, Cuddy, Yum, Geyer, Pleasure: "Differential expression of bcl2 protooncogene in neuroblastoma and other human tumor cell lines of neural origin." in: Cancer research, Vol. 51, Issue 24, pp. 6529-38, 1992

Williams: "Programmed cell death: apoptosis and oncogenesis." in: Cell, Vol. 65, Issue 7, pp. 1097-8, 1991

Pezzella, Tse, Cordell, Pulford, Gatter, Mason: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN967321) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN967321) Profile of permeabilized lymphocytes analyzed on a FACScan (BDIS, San Jose, CA) (Firs...
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN967321) Western blot analysis of Bcl-2 (Second Panel). Lysate from Jurkat cells was probed wi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN967321) Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using ...