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anti-Monkey BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
AA 41-54
168
71
63
54
50
44
23
23
23
19
17
15
14
14
13
13
13
13
12
12
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Pig (Porcine)
954
221
167
39
23
15
6
4
3
3
2
2
2
1
Host
Mouse
522
508
19
5
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
766
341
322
300
290
280
257
180
154
67
42
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN3026592
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN3026594 FACS IF IHC WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8

Similar anti-BCL2 Antibodies

Application / Reactivity Pig (Porcine) Monkey Human
Flow Cytometry (FACS) 22 Antibodies 2 Antibodies
Immunofluorescence (IF) 24 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 23 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Western Blotting (WB) 28 Antibodies 6 Antibodies
ELISA 3 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 42 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(168), (71), (63), (54), (50), (44), (23), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Pig (Porcine)
(954), (221), (167), (39), (23), (15), (6), (4), (3), (3), (2), (2), (2), (1)
Host Mouse
(522), (508), (19), (5), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(766), (341), (322), (300), (290), (280), (257), (180), (154), (67), (42), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Purity Protein G affinity chromatography
Immunogen A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Clone 8C8
Isotype IgG1, kappa

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Bcl-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).