anti-Monkey BCL2 antibody for Immunofluorescence

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • Bcl-2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
  • BCL-2
  • bcl-2
  • Bcl2
  • B-cell CLL/lymphoma 2
  • B cell leukemia/lymphoma 2
  • B-cell CLL/lymphoma 2 protein
  • B-cell leukemia/lymphoma 2
  • BCL2
  • LOC100224888
  • Bcl2
  • BCL-2
  • bcl2
Epitope
AA 41-54
164
73
64
59
41
33
25
25
24
20
18
15
15
15
13
13
13
11
8
7
6
5
5
5
5
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Monkey, Pig (Porcine)
997
252
211
53
27
22
18
17
5
4
4
3
2
2
2
2
Host
Mouse
544
514
19
6
2
2
2
Clonality
Monoclonal
Conjugate
This BCL2 antibody is un-conjugated
48
47
43
37
24
23
22
15
15
13
13
13
13
13
13
13
12
12
12
7
7
7
6
6
6
6
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
784
367
359
309
308
292
226
188
169
81
40
15
13
12
12
11
9
4
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN337080
$ 595.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN386113 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN3026592 FACS IF IHC WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8
1 ABIN386116 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN386117 FACS IF IHC (p) WB Biotin Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN386114 FACS IF IHC (p) WB Mouse IgG1 AA 41-54 Log in to see 8C8
1 ABIN2738523 ELISA FACS IF IHC (p) IP WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8
1 ABIN3026594 FACS IF IHC WB Mouse IgG1, kappa AA 41-54 Log in to see 8C8

Similar anti-BCL2 Antibodies

Application / Reactivity Pig (Porcine) Monkey Human
ELISA 23 Antibodies 7 Antibodies
Flow Cytometry (FACS) 30 Antibodies 8 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 32 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 22 Antibodies 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies 9 Antibodies
Immunoprecipitation (IP) 24 Antibodies 7 Antibodies
Western Blotting (WB) 40 Antibodies 17 Antibodies
Cell Culture (CC) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope AA 41-54
(164), (73), (64), (59), (41), (33), (25), (25), (24), (20), (18), (15), (15), (15), (13), (13), (13), (11), (8), (7), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Monkey, Pig (Porcine)
(997), (252), (211), (53), (27), (22), (18), (17), (5), (4), (4), (3), (2), (2), (2), (2)
Host Mouse
(544), (514), (19), (6), (2), (2), (2)
Clonality
Monoclonal
Conjugate This BCL2 antibody is un-conjugated
(48), (47), (43), (37), (24), (23), (22), (15), (15), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(784), (367), (359), (309), (308), (292), (226), (188), (169), (81), (40), (15), (13), (12), (12), (11), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human Bcl-2a at ~25kD. Species cross-reactivity: monkey. Does not react with mouse or rat.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%) Bovine (93%) Horse (86%).
Purification Protein G purified
Immunogen Synthetic peptide corresponding to residues 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein. Cellular Localization: Cytoplasm. MW of antigen: 25-26kD. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Dog, Cat, Pig (100%), Bovine (93%), Horse (86%).

Type of Immunogen: Synthetic peptide
Isotype IgG1

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BCL2 / Bcl-2 (BCL2 Antibody Abstract)
Background Name/Gene ID: BCL2
Family: Apoptosis

Synonyms: BCL2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, Bcl-2, PPP1R50
Gene ID 596
UniProt P10415
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling Images back to top
Application Notes Approved: ELISA, Flo, IF, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL for 2hrs at RT. Positive control: Tonsil or follicular lymphomas. Raji or HeLa cells. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4.
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C,-20 °C
Storage Comment +4°C or -20°C, Avoid repeated freezing and thawing.

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (AA 41-54) (ABIN337080) Human Spleen (formalin-fixed, paraffin-embedded) stained with BCL2 antibody ABIN33708...