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anti-Rat (Rattus) BCL2 antibody for Immunoprecipitation

Recommended BCL2 Antibody (supplied by: Log in to see )

Antigen
B-Cell CLL/lymphoma 2 (BCL2) Antibodies
  • AW986256
  • bcl-2
  • BCL-2
  • Bcl-2
  • Bcl2
  • C430015F12Rik
  • D630044D05Rik
  • D830018M01Rik
  • PPP1R50
Epitope
N-Term
169
71
62
54
50
44
23
23
23
19
17
15
14
14
13
13
13
13
12
12
7
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
954
220
166
40
23
15
7
4
3
3
2
2
2
1
Host
Rabbit
523
507
19
5
2
2
2
Clonality
Polyclonal
Conjugate
This BCL2 antibody is un-conjugated
47
45
40
36
24
23
19
14
13
13
13
13
13
13
13
13
12
12
12
12
7
6
6
6
6
6
6
3
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
766
341
322
301
291
280
256
180
154
67
42
11
10
10
8
8
5
1
1
1
1
1
Supplier
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Catalog No. ABIN222888
$ 304.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN968024 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.00089556724 ABIN968025 IF IP IHC WB Mouse IgG1 AA 49-179 Log in to see 7-Bcl 5
0.00089556724 ABIN256543 ICC IF IP WB Mouse IgG1 kappa N-Term, AA 61-76 Log in to see BCL-10C4 2
0.00089556724 ABIN317971 IHC (p) IP WB Rabbit pSer70 Log in to see Polyclonal
0.00089556724 ABIN126981 IHC (fro) IP WB Rabbit N-Term Log in to see Polyclonal
0.00089556724 ABIN264903 IF IP WB Sheep IgG Log in to see Polyclonal
0.00089556724 ABIN281585 FACS IP ICS WB Mouse IgG1 kappa Log in to see 10C4
0.00089556724 ABIN1584069 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.00089556724 ABIN2957874 IC IF IP IHC WB Rabbit pSer56 Log in to see Polyclonal
0.00089556724 ABIN184365 IHC (fro) IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN4283377 ICC IF IP WB DyLight 405LS Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283378 ICC IF IP WB DyLight 350 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283379 ICC IF IP WB DyLight 405 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283380 ICC IF IP WB DyLight 488 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283381 ICC IF IP WB PE Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283382 ICC IF IP WB PerCP Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283383 ICC IF IP WB Allophycocyanin Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283384 ICC IF IP WB DyLight 680 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283385 ICC IF IP WB DyLight 755 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4
0.00089556724 ABIN4283389 ICC IF IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 61-76, N-Term Log in to see BCL-10C4

Top referenced anti-BCL2 antibody for Immunoprecipitation

  • Chicken Monoclonal BCL2 Primary Antibody for IF, IP - ABIN968025 (5 Publications): Ayllón, Cayla, García, Roncal, Fernández, Albar, Martínez, Rebollo: Bcl-2 targets protein phosphatase 1 alpha to Bad. in Journal of immunology (Baltimore, Md. : 1950) 2001 (PubMed)

  • Chicken Monoclonal BCL2 Primary Antibody for IF, IP - ABIN968024 (5 Publications): Decary, Decesse, Ogryzko, Reed, Naguibneva, Harel-Bellan, Cremisi: The retinoblastoma protein binds the promoter of the survival gene bcl-2 and regulates its transcription in epithelial cells through transcription factor AP-2. in Molecular and cellular biology 2002 (PubMed)

  • Human Polyclonal BCL2 Primary Antibody for IP, IHC - ABIN222888 (4 Publications): Giuliani, Mioni, Altavilla, Leone, Bazzani, Minutoli, Bitto, Cainazzo, Marini, Zaffe, Botticelli, Pizzala, Savio, Necchi, Schiöth, Bertolini, Squadrito, Guarini: Both early and delayed treatment with melanocortin 4 receptor-stimulating melanocortins produces neuroprotection in cerebral ischemia. in Endocrinology 2006 (PubMed)

  • Mouse (Murine) Monoclonal BCL2 Primary Antibody for ICC, IF - ABIN256543 (2 Publications): Zuber, Rappaport, Luo, Wang, Chen, Vaseva, Shi, Weissmueller, Fellmann, Fellman, Taylor, Weissenboeck, Graeber, Kogan, Vakoc, Lowe: An integrated approach to dissecting oncogene addiction implicates a Myb-coordinated self-renewal program as essential for leukemia maintenance. in Genes & development 2011 (PubMed)

Similar anti-BCL2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 56 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 25 Antibodies 33 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 30 Antibodies
Intracellular Staining (ICS) 5 Antibodies
Western Blotting (WB) 97 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies

General

Antigen B-Cell CLL/lymphoma 2 (BCL2) Antibodies
Epitope N-Term
(169), (71), (62), (54), (50), (44), (23), (23), (23), (19), (17), (15), (14), (14), (13), (13), (13), (13), (12), (12), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(954), (220), (166), (40), (23), (15), (7), (4), (3), (3), (2), (2), (2), (1)
Host Rabbit
(523), (507), (19), (5), (2), (2), (2)
Clonality
Polyclonal
Conjugate This BCL2 antibody is un-conjugated
(47), (45), (40), (36), (24), (23), (19), (14), (13), (13), (13), (13), (13), (13), (13), (13), (12), (12), (12), (12), (7), (6), (6), (6), (6), (6), (6), (3), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(766), (341), (322), (301), (291), (280), (256), (180), (154), (67), (42), (11), (10), (10), (8), (8), (5), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-BCL2 Antibody

Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN222888) Images
Blocking Peptide Blocking peptide for this product available: ABIN412606
Specificity Detects a 25 kDa protein, corresponding to the apparent molecular weight of the ? isoform of Bcl-2 on SDS-PAGE immunoblots.
Purification Affinity purified
Immunogen Synthetic peptide corresponding to the N-terminus of human Bcl-2
Isotype IgG

Target Details BCL2

Product Details anti-BCL2 Antibody Application Details Handling References for anti-BCL2 Antibody (ABIN222888) Images back to top
Antigen
Alternative Name Bcl-2 (BCL2 Antibody Abstract)
Background Bcl-2 and its homologs belong to a family of proteins that either promotes or prevent apoptosis. Bcl-2 alpha and beta are the alternatively spliced isoforms of 25 kDa and 22 kDa mitochondrial integral membrane proteins that inhibit apoptosis.
Synonyms: BCL2, BCL 2
Gene ID 596
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BCL2 Antibody Target Details BCL2 Handling References for anti-BCL2 Antibody (ABIN222888) Images back to top
Application Notes Western blot analysis (0.5-4 μg/mL), Immunoprecipation (4-8 μg/mL), and Immunohistochemistry (10-20 μg/mL).
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details References for anti-BCL2 Antibody (ABIN222888) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-Bcl-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-BCL2 Antibody (ABIN222888)

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling Images back to top
Product cited in:

Eslami, Sharifi, Rahimi, Rahati: "Protective effect of telmisartan against oxidative damage induced by high glucose in neuronal PC12 cell." in: Neuroscience letters, 2013

Guérin, Cornut-Thibaut, Giscard-Destaing, Pouvreau, Guillemin, Aouacheria: "Subcellular dynamics of the maternal cell death regulator BCL2L10 in human preimplantation embryos." in: Human reproduction (Oxford, England), Vol. 28, Issue 3, pp. 729-39, 2013

Kiran Kumar, Prabhakara Rao, Noble, Weddington, McDowell, Rajanna, Bettaiya: "Lead-induced alteration of apoptotic proteins in different regions of adult rat brain." in: Toxicology letters, Vol. 184, Issue 1, pp. 56-60, 2008

Giuliani, Mioni, Altavilla, Leone, Bazzani, Minutoli, Bitto, Cainazzo, Marini, Zaffe, Botticelli, Pizzala, Savio, Necchi, Schiöth, Bertolini, Squadrito, Guarini: "Both early and delayed treatment with melanocortin 4 receptor-stimulating melanocortins produces neuroprotection in cerebral ischemia." in: Endocrinology, Vol. 147, Issue 3, pp. 1126-35, 2006

Images

Product Details anti-BCL2 Antibody Target Details BCL2 Application Details Handling References for anti-BCL2 Antibody (ABIN222888) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 antibody (B-Cell CLL/lymphoma 2) (N-Term) (ABIN222888) Western blot analysis of Bcl-2 expression in HL60 cell lysate.