You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Fruit Fly (Drosophila melanogaster) C-JUN antibody for Enzyme Immunoassay

Recommended C-JUN Antibody (supplied by: Log in to see )

Antigen
Jun Proto-Oncogene (JUN) Antibodies
  • AP-1
  • AP1
  • B230310J22Rik
  • Bsk
  • BSK
  • BSK/DJNK
  • c-jun
  • c-Jun
  • CG2275
  • CG5680
  • cJun
  • D-JNK
  • d-JRA
  • d-jun
  • d-Jun
  • D-Jun
  • D-jun
  • D-jun/Jra
  • D-junk
  • dAP-1
  • DBSK/JNK
  • DJNK
  • dJNK
  • DJNK/bsk
  • dJRA
  • dJra
  • DJUN
  • DJun
  • djun
  • dJun
  • dJUN
  • Djun
  • dm-Jun
  • Dmel\\CG2275
  • Dmel\\CG5680
  • fj36h07
  • JNK
  • Jnk
  • jnk
  • JNK/SAPK
  • JRA
  • jra
  • jun
  • Jun
  • JUN
  • Junc
  • Junk
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • p39
  • SAPKa
  • V
  • wu:fj36h07
  • zgc:65863
Epitope
N-Term
77
73
60
42
41
41
41
34
23
20
19
19
13
13
13
13
12
10
8
8
8
8
8
6
6
6
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster)
794
436
392
42
36
31
22
17
17
12
12
8
5
5
4
3
2
2
2
1
Host
Rabbit
702
116
2
2
Clonality
Polyclonal
Conjugate
This C-JUN antibody is un-conjugated
18
18
18
11
10
10
8
8
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
632
373
200
122
100
79
75
69
23
21
11
11
7
6
3
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN492934
$ 456.50
Plus shipping costs $45.00

Top referenced anti-C-JUN antibody for Enzyme Immunoassay

Similar anti-C-JUN Antibodies

Application / Reactivity Fruit Fly (Drosophila melanogaster)
Enzyme Immunoassay (EIA) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 10 Antibodies

General

Antigen Jun Proto-Oncogene (JUN) Antibodies
Epitope N-Term
(77), (73), (60), (42), (41), (41), (41), (34), (23), (20), (19), (19), (13), (13), (13), (13), (12), (10), (8), (8), (8), (8), (8), (6), (6), (6), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Fruit Fly (Drosophila melanogaster)
(794), (436), (392), (42), (36), (31), (22), (17), (17), (12), (12), (8), (5), (5), (4), (3), (2), (2), (2), (1)
Host Rabbit
(702), (116), (2), (2)
Clonality
Polyclonal
Conjugate This C-JUN antibody is un-conjugated
(18), (18), (18), (11), (10), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(632), (373), (200), (122), (100), (79), (75), (69), (23), (21), (11), (11), (7), (6), (3), (3), (2), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-C-JUN Antibody

Target Details C-JUN Application Details Handling References for anti-C-JUN Antibody (ABIN492934) Images
Specificity This antibody detects the Dfos and AP-1 complex in western blot positive control sample.
Cross-Reactivity (Details) Species reactivity (tested):Drosophila melanogaster
Purification Affinity chromatography
Immunogen Synthetic peptide

Target Details C-JUN

Product Details anti-C-JUN Antibody Application Details Handling References for anti-C-JUN Antibody (ABIN492934) Images back to top
Antigen
Alternative Name AP-1 / C-Jun (JUN Antibody Abstract)
Background The Dfos/kayak gene encodes a bZIP protein, DFos, required in a large variety of differentiation and morphogenetic processes throughout Drosophila development. Fos and Jun proteins homo- or heterodimerize to form functional AP-1 transcription factor. Mutation in these genes cause phenotypic mutations in Drosophila. Drosophila mutants lacking either Jun or Fos display indistinguishable dorsal open phenotypes, indicating an essential function of both Jun and Fos for embryonic dorsal closure (1). Phenotypic analysis of these mutants reveals that homodimers of Fos or of Jun cannot replace the function of the heterodimeric complex. This defect is not explained by the lower stability of homodimers as compared to heterodimers, because 'pseudo-homodimers' which are as stable as native Jun-Fos heterodimers cannot substitute for their function. Thus Jun and Fos play complementary roles that are both required for signal transduction and gene activation during dorsal closure. AP-1 transcription factor Dfos and Forkhead Box O transcription factor Foxo are also required downstream of Jun-N-terminal kinase signaling for the apoptotic response to UV-induced DNA damage in the developing Drosophila retina (2). Activation of JNK pathway is also involved along with puckered (puc) expression in wound healing of D. melanogaster wing imaginal disc wound healing and dorsal closure, the cells of the epidermis activates the AP-1 transcription factor comprised of DJUN and DFOS that, in turn, upregulates the expression of the dpp gene. stage-specific steroid-triggered programmed cell death of larval tissues during Drosophila metamorphosis induce the expression of several key genes of Rel/NF-Kappa B and AP-1 (3). Dfos is induced in a stage-specific manner, immediately before this tissue is destroyed while Djun is expressed for many hours before salivary gland cell death (3). Spatial and temporal regulation of the JNK signaling cascade may be a general mechanism that controls tissue remodeling during morphogenesis and wound healing. Djun and Dfos mRNA are continuously expressed and their abundance levels are transiently regulated by multiple signaling pathways, the peak response coming at 1-2 hours after perturbation. Djun and Dfos, the products of the Drosophila proto-oncongenes Djun and Dfos, are similar in size and sequence to their mammalian counterparts c-Jun and c-Fos and are related to their mammalian counterparts by their antigenic properties. The Dfos is more highly regulated than Djun which is only modulated. The receptor tyrosine kinase pathways positively regulate Dfos and Djun. The cAMP-mediated pathway positively regulates Dfos but negatively regulates Djun. The protein kinase C-activated pathway does not affect Djun whereas it negatively regulates Dfos (4).Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39
Gene ID 36057
NCBI Accession NP_476586
UniProt P18289
Research Area Transcription Factors
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-C-JUN Antibody Target Details C-JUN Handling References for anti-C-JUN Antibody (ABIN492934) Images back to top
Application Notes ELISA. Western blot: > 1: 1000. Immunoprecipitation: IMM & i. p pull-down assays: > 1: 250. Immunohistochemistry on frozen sections: < 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details References for anti-C-JUN Antibody (ABIN492934) Images back to top
Concentration 1.0 - 1.25 mg/mL
Buffer Stabilization buffer
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-C-JUN Antibody (ABIN492934)

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling Images back to top
Background publications

Luo, Puig, Hyun, Bohmann, Jasper: "Foxo and Fos regulate the decision between cell death and survival in response to UV irradiation." in: The EMBO journal, Vol. 26, Issue 2, pp. 380-90, 2007

Lehmann, Jiang, Ip, Thummel: "AP-1, but not NF-kappa B, is required for efficient steroid-triggered cell death in Drosophila." in: Cell death and differentiation, Vol. 9, Issue 5, pp. 581-90, 2002

Ciapponi, Bohmann: "An essential function of AP-1 heterodimers in Drosophila development." in: Mechanisms of development, Vol. 115, Issue 1-2, pp. 35-40, 2002

Xia, Goldstein: "Response of Djun and Dfos mRNA abundance to signal transduction pathways in cultured cells of Drosophila melanogaster." in: Molecular biology reports, Vol. 26, Issue 3, pp. 147-57, 1999