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anti-Human C-JUN antibody for Enzyme Immunoassay

Recommended C-JUN Antibody (supplied by: Log in to see )

Antigen
Jun Proto-Oncogene (JUN) Antibodies
  • AP-1
  • AP1
  • B230310J22Rik
  • Bsk
  • BSK
  • BSK/DJNK
  • c-jun
  • c-Jun
  • CG2275
  • CG5680
  • cJun
  • D-JNK
  • d-JRA
  • d-jun
  • d-Jun
  • D-Jun
  • D-jun
  • D-jun/Jra
  • D-junk
  • dAP-1
  • DBSK/JNK
  • DJNK
  • dJNK
  • DJNK/bsk
  • dJRA
  • dJra
  • DJUN
  • DJun
  • djun
  • dJun
  • dJUN
  • Djun
  • dm-Jun
  • Dmel\\CG2275
  • Dmel\\CG5680
  • fj36h07
  • JNK
  • Jnk
  • jnk
  • JNK/SAPK
  • JRA
  • jra
  • jun
  • Jun
  • JUN
  • Junc
  • Junk
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • p39
  • SAPKa
  • V
  • wu:fj36h07
  • zgc:65863
Epitope
pSer63
71
58
41
41
40
40
40
34
23
20
19
19
13
13
13
13
12
11
8
8
8
8
8
6
6
6
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
748
410
349
42
36
31
21
17
17
12
12
9
5
5
4
3
2
2
2
Host
Rabbit
678
95
2
2
Clonality
Polyclonal
Conjugate
This C-JUN antibody is un-conjugated
17
17
17
10
10
10
7
7
7
7
7
7
7
7
7
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
587
349
186
99
98
79
75
62
21
20
11
11
7
6
4
3
2
1
1
Supplier
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Catalog No. ABIN358110
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008896704 ABIN358111 EIA IHC (p) WB Rabbit Ig pSer63 Log in to see Polyclonal 5
0.0008896704 ABIN265356 EIA IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008896704 ABIN272013 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008896704 ABIN1105390 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4H9 1
0.0008896704 ABIN1105389 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5B1 1
0.0008896704 ABIN2716184 DB EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-C-JUN antibody for Enzyme Immunoassay

Similar anti-C-JUN Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
Dot Blot (DB) 57 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 172 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 102 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 79 Antibodies
Immunohistochemistry (IHC) 355 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 106 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Luminex Assay (LMNX) 6 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Jun Proto-Oncogene (JUN) Antibodies
Epitope pSer63
(71), (58), (41), (41), (40), (40), (40), (34), (23), (20), (19), (19), (13), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (6), (6), (6), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(748), (410), (349), (42), (36), (31), (21), (17), (17), (12), (12), (9), (5), (5), (4), (3), (2), (2), (2)
Host Rabbit
(678), (95), (2), (2)
Clonality
Polyclonal
Conjugate This C-JUN antibody is un-conjugated
(17), (17), (17), (10), (10), (10), (7), (7), (7), (7), (7), (7), (7), (7), (7)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(587), (349), (186), (99), (98), (79), (75), (62), (21), (20), (11), (11), (7), (6), (4), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-C-JUN Antibody

Target Details C-JUN Application Details Handling References for anti-C-JUN Antibody (ABIN358110) Images
Specificity This antibody detects AP-1/c-Jun pSer63.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.
Isotype Ig

Target Details C-JUN

Product Details anti-C-JUN Antibody Application Details Handling References for anti-C-JUN Antibody (ABIN358110) Images back to top
Antigen
Alternative Name AP-1 / C-Jun (JUN Antibody Abstract)
Background The gene for the cJun protein is the putative transforming gene of avian sarcoma virus 17. The protein is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene for this protein is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39
Molecular Weight 35676 Da
Gene ID 3725, 9606
UniProt P05412
Research Area Transcription Factors
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-C-JUN Antibody Target Details C-JUN Handling References for anti-C-JUN Antibody (ABIN358110) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details References for anti-C-JUN Antibody (ABIN358110) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-C-JUN Antibody (ABIN358110)

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling Images back to top
Background publications

Fang, Kerppola: "Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 41, pp. 14782-7, 2004

Wang, Li, Qi, Yuan, Ai, Zhu, Cao, Yang, Liu, Wu, Liu: "TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1." in: Biochemical and biophysical research communications, Vol. 323, Issue 1, pp. 9-16, 2004

Wehkamp, Harder, Wehkamp, Wehkamp-von Meissner, Schlee, Enders, Sonnenborn, Nuding, Bengmark, Fellermann, Schröder, Stange: "NF-kappaB- and AP-1-mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917: a novel effect of a probiotic bacterium." in: Infection and immunity, Vol. 72, Issue 10, pp. 5750-8, 2004

Gensch, Gallup, Sucher, Li, Gebremichael, Lemjabbar, Mengistab, Dasari, Hotchkiss, Harkema, Basbaum: "Tobacco smoke control of mucin production in lung cells requires oxygen radicals AP-1 and JNK." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39085-93, 2004

Fujioka, Niu, Schmidt, Sclabas, Peng, Uwagawa, Li, Evans, Abbruzzese, Chiao: "NF-kappaB and AP-1 connection: mechanism of NF-kappaB-dependent regulation of AP-1 activity." in: Molecular and cellular biology, Vol. 24, Issue 17, pp. 7806-19, 2004

Images

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling References for anti-C-JUN Antibody (ABIN358110) back to top
Supplier Images
 image for anti-C-JUN antibody (Jun Proto-Oncogene) (pSer63) (ABIN358110) anti-Jun Proto-Oncogene (JUN) (pSer63) antibody
 image for anti-C-JUN antibody (Jun Proto-Oncogene) (pSer63) (ABIN358110) anti-Jun Proto-Oncogene (JUN) (pSer63) antibody (Image 2)