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anti-Human C-JUN antibody for Enzyme Immunoassay

Recommended C-JUN Antibody (supplied by: Log in to see )

Antigen
Jun Proto-Oncogene (JUN) Antibodies
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • Jun
  • AP-1
  • JUN
  • B230310J22Rik
  • p39
  • Junc
  • c-jun
  • AP1
  • fj36h07
  • wu:fj36h07
  • zgc:65863
  • CG2275
  • D-Jun
  • D-jun
  • D-jun/Jra
  • DJUN
  • DJun
  • Djun
  • Dmel\\CG2275
  • JRA
  • V
  • cJun
  • d-JRA
  • d-Jun
  • d-jun
  • dAP-1
  • dJRA
  • dJUN
  • dJra
  • dJun
  • djun
  • dm-Jun
  • jra
  • jun
  • l(2)46Ef
  • l(2)IA109
  • l(2R)IA109
  • jun oncogene
  • basket
  • C-JUN protein
  • c-Jun protein
  • c-jun transcription factor
  • cyclin-dependent kinase 5, regulatory subunit 2 (p39)
  • Jun oncogene
  • jun proto-oncogene
  • Jun-related antigen
  • jun
  • bsk
  • C-JUN
  • Cdk5r2
  • Jun
  • JUN
  • Jra
Epitope
pSer63
75
70
48
44
43
42
40
30
22
20
20
19
17
15
15
15
12
12
8
8
8
8
8
7
6
6
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
843
571
515
71
62
52
44
41
35
34
29
17
11
10
6
6
4
3
3
2
2
1
1
Host
Rabbit
736
125
3
2
1
Clonality
Polyclonal
Conjugate
This C-JUN antibody is un-conjugated
18
18
18
11
10
10
8
8
7
7
7
7
7
7
7
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
684
382
227
154
106
93
87
75
25
22
13
11
10
7
6
4
2
2
1
1
Supplier
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Catalog No. ABIN358111
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358110 EIA IHC (p) WB Rabbit Ig pSer63 Log in to see Polyclonal 5
1 ABIN265356 EIA IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN272013 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1105389 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5B1 1
1 ABIN1105390 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4H9 1
1 ABIN2716184 DB EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-C-JUN antibody for Enzyme Immunoassay

Similar anti-C-JUN Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 3 Antibodies
Dot Blot (DB) 56 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 212 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunochromatography (IC) 9 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 106 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 93 Antibodies
Immunohistochemistry (IHC) 382 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 155 Antibodies
Immunoprecipitation (IP) 85 Antibodies
Luminex Assay (LMNX) 6 Antibodies

General

Antigen Jun Proto-Oncogene (JUN) Antibodies
Epitope pSer63
(75), (70), (48), (44), (43), (42), (40), (30), (22), (20), (20), (19), (17), (15), (15), (15), (12), (12), (8), (8), (8), (8), (8), (7), (6), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(843), (571), (515), (71), (62), (52), (44), (41), (35), (34), (29), (17), (11), (10), (6), (6), (4), (3), (3), (2), (2), (1), (1)
Host Rabbit
(736), (125), (3), (2), (1)
Clonality
Polyclonal
Conjugate This C-JUN antibody is un-conjugated
(18), (18), (18), (11), (10), (10), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(684), (382), (227), (154), (106), (93), (87), (75), (25), (22), (13), (11), (10), (7), (6), (4), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-C-JUN Antibody

Target Details C-JUN Application Details Handling References for anti-C-JUN Antibody (ABIN358111) Images
Specificity This antibody detects AP-1/c-Jun pSer63. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.
Isotype Ig

Target Details C-JUN

Product Details anti-C-JUN Antibody Application Details Handling References for anti-C-JUN Antibody (ABIN358111) Images back to top
Antigen
Alternative Name AP-1 / C-Jun (JUN Antibody Abstract)
Background The gene for cJun is the putative transforming gene of avian sarcoma virus 17. The cJun protein is a transcription factor highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene maps to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39
Molecular Weight 35676 Da
Gene ID 3725, 9606
UniProt P05412
Research Area Transcription Factors
Pathways MAPK Signaling, RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction

Application Details

Product Details anti-C-JUN Antibody Target Details C-JUN Handling References for anti-C-JUN Antibody (ABIN358111) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details References for anti-C-JUN Antibody (ABIN358111) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-C-JUN Antibody (ABIN358111)

Product Details anti-C-JUN Antibody Target Details C-JUN Application Details Handling Images back to top
Background publications

Cheng, Perkins, Yeh: "Differential regulation of c-Jun-dependent transcription by SUMO-specific proteases." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14492-8, 2005

Quan, He, Voorhees, Fisher: "Ultraviolet irradiation induces Smad7 via induction of transcription factor AP-1 in human skin fibroblasts." in: The Journal of biological chemistry, Vol. 280, Issue 9, pp. 8079-85, 2005

Bladh, Lidén, Dahlman-Wright, Reimers, Nilsson, Okret et al.: "Identification of endogenous glucocorticoid repressed genes differentially regulated by a glucocorticoid receptor mutant able to separate between nuclear factor-kappaB and activator protein-1 ..." in: Molecular pharmacology, Vol. 67, Issue 3, pp. 815-26, 2005

DeNardo, Kim, Hilsenbeck, Cuba, Tsimelzon, Brown: "Global gene expression analysis of estrogen receptor transcription factor cross talk in breast cancer: identification of estrogen-induced/activator protein-1-dependent genes." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 2, pp. 362-78, 2005

Cheung, Acevedo, Cole, Kraus: "Altered pharmacology and distinct coactivator usage for estrogen receptor-dependent transcription through activating protein-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 3, pp. 559-64, 2005

Images

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Supplier Images
 image for anti-C-JUN antibody (Jun Proto-Oncogene) (pSer63) (ABIN358111) anti-Jun Proto-Oncogene (JUN) (pSer63) antibody
 image for anti-C-JUN antibody (Jun Proto-Oncogene) (pSer63) (ABIN358111) anti-Jun Proto-Oncogene (JUN) (pSer63) antibody (Image 2)