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|Antigen||Calnexin (CANX) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This Calnexin antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Calnexin AntibodyTarget Details Calnexin Application Details Handling Images
|Specificity||This antibody recognizes Calnexin.|
Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse.
|Purification||Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX|
Target Details CalnexinProduct Details anti-Calnexin Antibody Application Details Handling Images back to top
|Alternative Name||Calnexin (CANX Antibody Abstract)|
|Background||Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90|
|Pathways||MAPK Signaling, Thyroid Hormone Synthesis|
Application DetailsProduct Details anti-Calnexin Antibody Target Details Calnexin Handling Images back to top
Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Calnexin Antibody Target Details Calnexin Application Details Images back to top
|Buffer||Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|