You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Calnexin antibody for Enzyme Immunoassay

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • 1110069N15Rik
  • AI988026
  • CANX
  • canx
  • CG11958
  • clgn
  • clnx
  • Cnx
  • CNX
  • cnx
  • D11Ertd153e
  • Dmel\CG11958
  • IP90
  • P90
  • PP90
  • wu:fe06b12
  • zgc:63524
Epitope
Internal Region
101
26
18
13
12
10
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
313
177
171
98
91
86
85
84
84
82
82
81
77
52
37
21
12
9
5
4
4
4
4
4
2
2
1
1
1
Host
Goat
223
87
23
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
12
10
10
8
7
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
308
207
162
145
126
108
84
72
11
9
4
3
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN401659
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854274 ABIN782342 EIA IHC (p) WB Mouse IgG2b AA 504-593 Log in to see 1D4
0.0008854274 ABIN1105640 EIA IF WB Mouse IgG2b Log in to see 3H4A7 2
0.0008854274 ABIN614430 EIA IF IHC (p) WB Mouse IgG2b Log in to see 3H4A7
0.0008854274 ABIN2561540 EIA IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal

Top referenced anti-Calnexin antibody for Enzyme Immunoassay

Similar anti-Calnexin Antibodies

Application / Reactivity Human Cow (Bovine) Mouse (Murine) Pig (Porcine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 291 Antibodies 91 Antibodies 164 Antibodies 86 Antibodies 159 Antibodies 97 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 99 Antibodies 57 Antibodies 74 Antibodies 55 Antibodies 74 Antibodies 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies 122 Antibodies 62 Antibodies 20 Antibodies 61 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 203 Antibodies

General

Antigen Calnexin (CANX) Antibodies
Epitope Internal Region
(101), (26), (18), (13), (12), (10), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(313), (177), (171), (98), (91), (86), (85), (84), (84), (82), (82), (81), (77), (52), (37), (21), (12), (9), (5), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1)
Host Goat
(223), (87), (23)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(12), (10), (10), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(308), (207), (162), (145), (126), (108), (84), (72), (11), (9), (4), (3), (3), (2), (2), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling References for anti-Calnexin Antibody (ABIN401659) Images
Sequence c-SKTPELNLDQ FHDKT
Specificity This antibody recognizes Calnexin.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling References for anti-Calnexin Antibody (ABIN401659) Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene ID 821
NCBI Accession NP_001737
UniProt P27824
Pathways MAPK Signaling

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling References for anti-Calnexin Antibody (ABIN401659) Images back to top
Application Notes Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details References for anti-Calnexin Antibody (ABIN401659) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Calnexin Antibody (ABIN401659)

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling Images back to top
Background publications

Rosser, Grove, Chen, Cyr: "Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2." in: Molecular biology of the cell, Vol. 19, Issue 11, pp. 4570-9, 2008

Images

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling References for anti-Calnexin Antibody (ABIN401659) back to top
Supplier Images
 image for anti-Calnexin antibody (CANX) (Internal Region) (ABIN401659) anti-Calnexin (CANX) (Internal Region) antibody
 image for anti-Calnexin antibody (CANX) (Internal Region) (ABIN401659) anti-Calnexin (CANX) (Internal Region) antibody (Image 2)
 image for anti-Calnexin antibody (CANX) (Internal Region) (ABIN401659) anti-Calnexin (CANX) (Internal Region) antibody (Image 3)