anti-Rat (Rattus) Calnexin antibody for Immunofluorescence

Recommended Calnexin Antibody (supplied by: Log in to see )

Antigen
Calnexin (CANX) Antibodies
  • zgc:63524
  • wu:fe06b12
  • clgn
  • clnx
  • cnx
  • CANX
  • CNX
  • IP90
  • P90
  • canx
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • PP90
  • CG11958
  • Dmel\CG11958
  • calnexin
  • Calnexin 99A
  • canx
  • CANX
  • CNX
  • LOC100283947
  • LOC100304606
  • canx-a
  • Canx
  • Cnx99A
Epitope
Internal Region, AA 25-100
103
44
19
14
14
12
10
8
5
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Bird (Avian), Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish
382
241
228
134
124
118
116
112
111
106
106
105
97
69
58
19
15
12
8
5
4
4
4
4
3
2
2
1
1
1
1
Host
Rabbit
242
132
29
Clonality
Polyclonal
Conjugate
This Calnexin antibody is un-conjugated
15
14
12
11
9
8
8
7
7
7
7
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
368
235
221
199
155
124
98
80
13
9
8
6
3
3
2
2
2
2
1
1
Supplier
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Catalog No. ABIN151464
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.009983 ABIN1531305 IF IHC ELISA WB Rabbit IgG AA 543-592, pSer583 Log in to see Polyclonal
12.009983 ABIN1532324 IF IHC ELISA WB Rabbit IgG AA 543-592 Log in to see Polyclonal
12.009983 ABIN151474 FACS ICC IF IHC IHC (p) IP SimWes WB Rabbit C-Term, AA 550-591 Log in to see Polyclonal 4
12.009983 ABIN152677 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see AF18 3
12.009983 ABIN2855124 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
12.009983 ABIN2704566 IC IF IP IHC WB Rabbit C-Term, pSer583 Log in to see Polyclonal
12.009983 ABIN2704567 IC IF IP IHC WB Rabbit C-Term Log in to see Polyclonal
12.009983 ABIN1440007 IF WB Goat IgG C-Term Log in to see Polyclonal 1
12.009983 ABIN1440005 IF WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN361782 ICC FACS IF IP IHC WB Rabbit C-Term Log in to see Polyclonal 7
1 ABIN271924 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4287258 ELISA ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN361827 ICC IF IHC WB Rabbit C-Term Log in to see Polyclonal 11
1 ABIN2481643 ICC FACS IF IP IHC WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481644 ICC FACS IF IP IHC WB PerCP Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481631 ICC FACS IF IP IHC WB Atto 488 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481632 ICC FACS IF IP IHC WB Atto 565 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481636 ICC FACS IF IP IHC WB Atto 680 Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481640 ICC FACS IF IP IHC WB Biotin Rabbit C-Term Log in to see Polyclonal 7
1 ABIN2481639 ICC FACS IF IP IHC WB APC Rabbit C-Term Log in to see Polyclonal 7

General

Antigen Calnexin (CANX) Antibodies
Epitope Internal Region, AA 25-100
(103), (44), (19), (14), (14), (12), (10), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bird (Avian), Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish
(382), (241), (228), (134), (124), (118), (116), (112), (111), (106), (106), (105), (97), (69), (58), (19), (15), (12), (8), (5), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(242), (132), (29)
Clonality
Polyclonal
Conjugate This Calnexin antibody is un-conjugated
(15), (14), (12), (11), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(368), (235), (221), (199), (155), (124), (98), (80), (13), (9), (8), (6), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 9 references available
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Product Details anti-Calnexin Antibody

Target Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151464) Images
Characteristics Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]

Target Details Calnexin

Product Details anti-Calnexin Antibody Application Details Handling References for anti-Calnexin antibody (ABIN151464) Images back to top
Antigen
Alternative Name Calnexin (CANX Antibody Abstract)
Background Gene Symbol: CANX
Molecular Weight Theoretical MW: 97 kDa
Gene ID 821
UniProt P24643
Pathways MAPK Signaling, Thyroid Hormone Synthesis

Application Details

Product Details anti-Calnexin Antibody Target Details Calnexin Handling References for anti-Calnexin antibody (ABIN151464) Images back to top
Application Notes Western Blot 2 μg/mL, Simple Western 1:25, Immunohistochemistry 1:40, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:40This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocol Specific for CANX antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details References for anti-Calnexin antibody (ABIN151464) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.5 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Calnexin antibody (ABIN151464)

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling Images back to top
Product cited in:

Li, Subrahmanyan, Smith, Yu, Zaidi, Choi, Mane, Nelson-Williams, Bahjati, Kazemi, Hashemi, Fathzadeh, Narayanan, Tian, Montazeri, Mani, Begleiter, Coon, Lynch, Olson, Zhao, Ruland, Lifton, Mani: "Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus." in: American journal of human genetics, Vol. 98, Issue 6, pp. 1082-91, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Xu, Li, Zalzala, Xu, Gonzalez, Adorini, Lee, Yin, Zhang: "Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice." in: Hepatology (Baltimore, Md.), Vol. 64, Issue 4, pp. 1072-85, 2016

Huynh, VonMoss, Smith, Rahman, Felemban, Zuo, Rody, McHugh, Holliday: "Characterization of Regulatory Extracellular Vesicles from Osteoclasts." in: Journal of dental research, Vol. 95, Issue 6, pp. 673-9, 2016 Method employed by authors: Immunoprecipitation (IP)

Smagris, Gilyard, BasuRay, Cohen, Hobbs: "Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins." in: The Journal of biological chemistry, Vol. 291, Issue 20, pp. 10659-76, 2016

Riemersma, Sandrock, Boltje, Büll, Heise, Ashikov, Adema, van Bokhoven, Lefeber: "Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid." in: Human molecular genetics, Vol. 24, Issue 8, pp. 2241-6, 2015 (Sample species: Human). Further details: Western Blotting

McFarlane, Cantoria, Linden, January, Liang, Engelking: "Scap is required for sterol synthesis and crypt growth in intestinal mucosa." in: Journal of lipid research, Vol. 56, Issue 8, pp. 1560-71, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yuen, Webb, Chan, Thisse, Thisse, Miller: "Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development." in: Biochimica et biophysica acta, Vol. 1833, Issue 7, pp. 1641-56, 2013 (Sample species: Zebrafish (Danio rerio)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Saeki, Sato, Ito, Ikeda, Kanamoto et al.: "Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport ..." in: BMC physiology, Vol. 13, pp. 4, 2013 (Sample species: Pig (Porcine)). Further details: Western Blotting

Tian, Hoffman, Ten Hagen: "O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components." in: Nature communications, Vol. 3, pp. 869, 2012 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Calnexin Antibody Target Details Calnexin Application Details Handling References for anti-Calnexin antibody (ABIN151464) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody
Immunohistochemistry (IHC) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (Image 2)
Immunofluorescence (IF) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) Immunocytochemistry/Immunofluorescence: Calnexin Antibody [ABIN151464] - Calnexin ant...
Immunohistochemistry (IHC) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) Immunohistochemistry: Calnexin Antibody [ABIN151464] - IHC analysis of CANX in mouse ...
Western Blotting (WB) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) Western Blot: Calnexin Antibody [ABIN151464] - WB analysis of CANX in cell lysates as...
Western Blotting (WB) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) Western Blot: Calnexin Antibody [ABIN151464] - A dilution of 1/2000 used in TBS with ...
Simple Western (SimWes) image for anti-Calnexin (CANX) (AA 25-100), (Internal Region) antibody (ABIN151464) Simple Western: Calnexin Antibody [ABIN151464] - Simple Western lane view shows a spe...