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anti-Rat (Rattus) Cortactin antibody for Immunoprecipitation

Recommended Cortactin Antibody (supplied by: Log in to see )

Antigen
Cortactin (CTTN) Antibodies
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Epitope
N-Term
28
26
17
15
14
12
9
7
6
6
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
180
106
60
8
7
5
3
2
2
1
1
1
1
1
1
Host
Rabbit
166
25
15
Clonality
Monoclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
171
89
85
22
16
9
7
3
3
2
1
1
1
Supplier
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Available images

Catalog No. ABIN189263
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Similar anti-Cortactin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 27 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 6 Antibodies
Immunofluorescence (IF) 9 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 27 Antibodies 51 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 14 Antibodies
Immunoprecipitation (IP) 1 Antibodies 4 Antibodies
Western Blotting (WB) 57 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Cortactin (CTTN) Antibodies
Epitope N-Term
(28), (26), (17), (15), (14), (12), (9), (7), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(180), (106), (60), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(166), (25), (15)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cortactin antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(171), (89), (85), (22), (16), (9), (7), (3), (3), (2), (1), (1), (1)
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Product Details anti-Cortactin Antibody

Target Details Cortactin Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near the N-terminus of human Cortactin was used as an immunogen.
Clone EP1922Y
Isotype IgG

Target Details Cortactin

Product Details anti-Cortactin Antibody Application Details Handling Images back to top
Antigen
Background Immunocytochemistry reveals that in epithelial cells, the human cortactin protein islocalized mainly in the cytoplasm and, to a very low extent, in protruding leadinglamellae of the cell. However, in carcinoma cells that constitutively overexpress cortactinaccumulates in the podosome-like adherens junctions associated with thecell-substratum contact sites. Overexpression and concomitant accumulation of theCortactin protein in the cell-substratum contact sites might, therefore, contribute to theinvasive potential of these tumor cells (1). In cells derived from breast carcinomas andsquamous carcinomas of the head and neck, DNA amplification of a particular tyrosinekinase substrate region results in overexpression of cortactin. Overexpression isaccompanied by a partial redistribution of cortactin from the cytoplasm into cell-matrixcontact sites (2).
Gene Symbol: CTTN
Gene ID 2017, 60465
Pathways MAPK Signaling

Application Details

Product Details anti-Cortactin Antibody Target Details Cortactin Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 80 kDa.
Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:40, Western Blot 1:50000-100000
Protocol Protocol specific for Cortactin Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (N-Term) (ABIN189263) Western blot analysis on HeLa cell lysate using anti-Cortactin RabMAb (ABIN189263), d...
Immunohistochemistry (IHC) image for anti-Cortactin antibody (CTTN) (N-Term) (ABIN189263) Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-C...
Immunofluorescence (IF) image for anti-Cortactin antibody (CTTN) (N-Term) (ABIN189263) Immunofluorescent staining of MCF7 cells using anti-Cortactin RabMAb