|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||27 Antibodies||37 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||6 Antibodies|
|Immunofluorescence (IF)||9 Antibodies||12 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||27 Antibodies||51 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||14 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies||4 Antibodies|
|Western Blotting (WB)||57 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||Cortactin (CTTN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cortactin antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-Cortactin AntibodyTarget Details Cortactin Application Details Handling Images
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near the N-terminus of human Cortactin was used as an immunogen.|
Target Details CortactinProduct Details anti-Cortactin Antibody Application Details Handling Images back to top
Immunocytochemistry reveals that in epithelial cells, the human cortactin protein islocalized mainly in the cytoplasm and, to a very low extent, in protruding leadinglamellae of the cell. However, in carcinoma cells that constitutively overexpress cortactinaccumulates in the podosome-like adherens junctions associated with thecell-substratum contact sites. Overexpression and concomitant accumulation of theCortactin protein in the cell-substratum contact sites might, therefore, contribute to theinvasive potential of these tumor cells (1). In cells derived from breast carcinomas andsquamous carcinomas of the head and neck, DNA amplification of a particular tyrosinekinase substrate region results in overexpression of cortactin. Overexpression isaccompanied by a partial redistribution of cortactin from the cytoplasm into cell-matrixcontact sites (2).
Gene Symbol: CTTN
|Gene ID||2017, 60465|
Application DetailsProduct Details anti-Cortactin Antibody Target Details Cortactin Handling Images back to top
In Western blot this antibody detects a band at approximately 80 kDa.
Recommended dilutions: Flow Cytometry 1:10, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:40, Western Blot 1:50000-100000
Protocol specific for Cortactin Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling back to top