|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Western Blotting (WB)||192 Antibodies||90 Antibodies||91 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies||27 Antibodies||25 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||32 Antibodies||22 Antibodies||18 Antibodies|
|Immunohistochemistry (IHC)||128 Antibodies||79 Antibodies||71 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||36 Antibodies||36 Antibodies||36 Antibodies|
|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Dot Blot (DB), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN265095) Images
|Specificity||This antibody reacts to ELK-1 (pSer383).|
|Immunogen||Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Ser383 of human Elk-1|
Target Details ELK1Product Details anti-ELK1 Antibody Application Details Handling References for anti-ELK1 Antibody (ABIN265095) Images back to top
|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
|Background||Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors. Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1. Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus.Synonyms: ETS domain-containing protein Elk-1, Elk-1|
|Research Area||Cancer, Transcription Factors|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling References for anti-ELK1 Antibody (ABIN265095) Images back to top
Western Blot: 1: 1000. Dot Blot: 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details References for anti-ELK1 Antibody (ABIN265095) Images back to top
|Buffer||HEPES ( pH 7.5) solution containing 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Ship on dry ice. Store (in aliquots) at -20 °C to -70 °C.|
References for anti-ELK1 Antibody (ABIN265095)Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling Images back to top
Li, Yang, Maeda et al.: "MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300." in: The EMBO journal, Vol. 22, Issue 2, pp. 281-91, 2003 (PubMed).
Hill, Treisman: "Transcriptional regulation by extracellular signals: mechanisms and specificity." in: Cell, Vol. 80, Issue 2, pp. 199-211, 1995 (PubMed).
ImagesProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN265095) back to top