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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Zebrafish (Danio rerio)||Non Human Primates|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||3 Antibodies||3 Antibodies||1 Antibodies|
|Western Blotting (WB)||236 Antibodies||109 Antibodies||117 Antibodies||19 Antibodies||3 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||42 Antibodies||41 Antibodies||35 Antibodies|
|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|2 references available|
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Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN372618) Images
|Specificity||Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.|
Species reactivity (expected):Human, Canine, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1.|
Target Details ELK1Product Details anti-ELK1 Antibody Application Details Handling References for anti-ELK1 Antibody (ABIN372618) Images back to top
|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
|Background||Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).Synonyms: ETS domain-containing protein Elk-1, Elk-1|
|Research Area||Cancer, Transcription Factors|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling References for anti-ELK1 Antibody (ABIN372618) Images back to top
Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details References for anti-ELK1 Antibody (ABIN372618) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-ELK1 Antibody (ABIN372618)Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling Images back to top
Li, Yang, Maeda, Sladek, Sharrocks, Martins-Green: "MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300." in: The EMBO journal, Vol. 22, Issue 2, pp. 281-91, 2003
Hill, Treisman: "Transcriptional regulation by extracellular signals: mechanisms and specificity." in: Cell, Vol. 80, Issue 2, pp. 199-211, 1995
ImagesProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN372618) back to top