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|Application / Reactivity||Human|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||16 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||43 Antibodies|
|Immunohistochemistry (IHC)||74 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||42 Antibodies|
|Immunoprecipitation (IP)||38 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Luminex Assay (LMNX)||5 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||216 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Epitope||AA 316-345, C-Term Alternatives|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN1882175) Images
|Predicted Reactivity||Cow (Bovine),Mouse (Murine),Rat (Rattus)|
|Purification||This antibody is purified through a protein A column, followed by peptide affinity purification. Purified Rabbit Polyclonal Antibody (Pab)|
|Immunogen||This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MAPK1.|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN1882175) Images back to top
|Alternative Name||MAPK1 (MAPK1 Antibody Abstract)|
MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Synonyms: MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2
|NCBI Accession||NP_002736, NP_620407|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN1882175) Images back to top
|Application Notes||WB: 1:1000,IHC (p): 1:50-100,FACS: 1:10-50|
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN1882175) Images back to top
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
References for anti-ERK2 Antibody (ABIN1882175)Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
|Product cited in:||
Lou, Xie, Zhang et al.: "Nek2A specifies the centrosomal localization of Erk2." in: Biochemical and biophysical research communications, Vol. 321, Issue 2, pp. 495-501, 2004 (PubMed).
Mukherjee, Huang, Petkova et al.: "Trypanosoma cruzi infection activates extracellular signal-regulated kinase in cultured endothelial and smooth muscle cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5274-82, 2004 (PubMed).
Huang, Chang, Liu: "Basic fibroblast growth factor antagonizes activin A-mediated growth inhibition and hemoglobin synthesis in K562 cells by activating ERK1/2 and deactivating p38 MAP kinase." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1247-52, 2004 (PubMed).
Munshi, Wu, Mukhopadhyay et al.: "Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ..." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39042-50, 2004 (PubMed).
Mitsushima, Suwa, Amachi et al.: "Extracellular signal-regulated kinase activated by epidermal growth factor and cell adhesion interacts with and phosphorylates vinexin." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34570-7, 2004 (PubMed).
ImagesProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN1882175) back to top