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|Application / Reactivity||Human|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Electron Microscopy (EM)||1 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||21 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||50 Antibodies|
|Immunofluorescence (IF)||82 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||89 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||79 Antibodies|
|Immunoprecipitation (IP)||56 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling Images
|Specificity||This antibody recognizes Extracellular Signal-Regulated Kinase 2 (MAPK1/ERK2).|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Characteristics||Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2|
|Immunogen||Ni-NTA purified full length recombinant ERK2 expressed in E. coli strain BL21 (DE3).|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling Images back to top
|Alternative Name||ERK2 (MAPK1 Antibody Abstract)|
|Background||ERK2 is activated by MEK2, which phosphorylates neighbouring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK|
|Research Area||Stem Cells, Signaling, Inflammation|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling Images back to top
ELISA: 1/10000. Western blot: 1/500-1/2000. Immunohistochemistry on Frozen Sections: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Images back to top
|Buffer||PBS containing 0.01 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling back to top