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anti-Human ERK2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Epitope
Middle Region
61
25
15
9
8
8
7
5
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
256
78
65
16
14
7
5
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
152
107
16
3
1
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
6
5
5
5
4
4
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
240
106
79
49
42
41
16
13
9
7
5
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN359461
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34458184 ABIN359631 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.34458184 ABIN305764 IHC (fro) IHC (p) ELISA WB Mouse IgG2a Log in to see 4C11C11C4
0.00089488085 ABIN391367 FACS IHC (p) WB Rabbit Ig AA 154-183, Center Log in to see Polyclonal 3
0.00089488085 ABIN391720 IHC (p) WB Rabbit Ig AA 254-285, C-Term Log in to see Polyclonal 2
0.00089488085 ABIN1882175 FACS IHC (p) WB Rabbit AA 316-345, C-Term Log in to see Polyclonal 5
0.00089488085 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
0.00089488085 ABIN302975 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089488085 ABIN1687923 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.00089488085 ABIN400733 EIA IHC (fro) IHC (p) WB Mouse IgG2a Log in to see 4C11C11C4
0.00089488085 ABIN1687922 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.00089488085 ABIN373411 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.00089488085 ABIN1499293 IHC (p) WB Mouse IgG2a Log in to see 9H1
0.00089488085 ABIN1499291 IHC (p) IP WB Mouse IgG1 Log in to see 9E9
0.00089488085 ABIN1499292 IHC (p) WB Mouse IgG1 Log in to see 6E5
0.00089488085 ABIN1499289 IF IHC (p) IP WB Mouse IgG1 Log in to see 4C2
0.00089488085 ABIN1102946 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00089488085 ABIN3044305 IHC (p) WB Rabbit IgG AA 171-184, N-Term Log in to see Polyclonal
0.00089488085 ABIN1499287 FACS IHC (p) IP WB Mouse IgG2a Log in to see 6F8
0.00089488085 ABIN438932 IHC (p) IHC PLA WB Rabbit IgG Log in to see Polyclonal
0.00089488085 ABIN258758 ICC IHC (fro) IF IHC (p) IHC ELISA WB Mouse IgG2a Log in to see 4C11

Top referenced anti-ERK2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

  • Human Polyclonal ERK2 Primary Antibody for FACS, IHC (p) - ABIN1882175 (5 Publications): Munshi, Wu, Mukhopadhyay, Ottaviano, Sassano, Koblinski, Platanias, Stack et al.: Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ... in The Journal of biological chemistry 2004 (PubMed)

Similar anti-ERK2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 100 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 38 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 222 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope Middle Region
(61), (25), (15), (9), (8), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(256), (78), (65), (16), (14), (7), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(152), (107), (16), (3), (1)
Clonality
Polyclonal
Conjugate This ERK2 antibody is un-conjugated
(6), (5), (5), (5), (4), (4), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(240), (106), (79), (49), (42), (41), (16), (13), (9), (7), (5), (3), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN359461) Images
Specificity This antibody detects ERK2 (MAPK1) at center.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MAPK1 Antibody.
Isotype Ig

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN359461) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molecular Weight 41390 Da
Gene ID 5594, 9606
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN359461) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 250 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN359461) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-ERK2 Antibody (ABIN359461)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Background publications

Lou, Xie, Zhang, Yao, Luo, Wang, Shi, Yao: "Nek2A specifies the centrosomal localization of Erk2." in: Biochemical and biophysical research communications, Vol. 321, Issue 2, pp. 495-501, 2004

Mitsushima, Suwa, Amachi, Ueda, Kioka: "Extracellular signal-regulated kinase activated by epidermal growth factor and cell adhesion interacts with and phosphorylates vinexin." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34570-7, 2004

Munshi, Wu, Mukhopadhyay, Ottaviano, Sassano, Koblinski, Platanias, Stack et al.: "Differential regulation of membrane type 1-matrix metalloproteinase activity by ERK 1/2- and p38 MAPK-modulated tissue inhibitor of metalloproteinases 2 expression controls transforming growth ..." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 39042-50, 2004

Mukherjee, Huang, Petkova, Albanese, Pestell, Braunstein, Christ, Wittner, Lisanti, Berman, Weiss, Tanowitz: "Trypanosoma cruzi infection activates extracellular signal-regulated kinase in cultured endothelial and smooth muscle cells." in: Infection and immunity, Vol. 72, Issue 9, pp. 5274-82, 2004

Huang, Chang, Liu: "Basic fibroblast growth factor antagonizes activin A-mediated growth inhibition and hemoglobin synthesis in K562 cells by activating ERK1/2 and deactivating p38 MAP kinase." in: Biochemical and biophysical research communications, Vol. 320, Issue 4, pp. 1247-52, 2004

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN359461) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (Middle Region) (ABIN359461) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAPK1 A...
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (Middle Region) (ABIN359461) Western blot analysis of anti-MAPK1 Antibody (Center) Pab in Hela, 293, and HepG2 cel...