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anti-Mouse (Murine) ERK2 antibody for Flow Cytometry

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Reactivity
Human, Mouse (Murine)
327
107
72
36
17
7
5
3
3
2
2
2
1
1
1
1
Host
Mouse
165
161
18
3
2
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
302
143
91
82
70
53
51
25
18
10
5
3
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2869484
$ 464.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2666104 FACS Rabbit IgG Log in to see Polyclonal 1
1 ABIN2470904 FACS IHC ELISA WB Mouse IgG2b Log in to see 3F8
1 ABIN4903606 FACS IF IHC WB Mouse IgG Log in to see

Top referenced anti-ERK2 antibody for Flow Cytometry

Similar anti-ERK2 Antibodies

Application / Reactivity Mouse (Murine) Human
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 45 Antibodies 136 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 95 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Reactivity Human, Mouse (Murine)
(327), (107), (72), (36), (17), (7), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(165), (161), (18), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(8), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(302), (143), (91), (82), (70), (53), (51), (25), (18), (10), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN2869484) Images
Purpose Purified Mouse Monoclonal Antibody
Characteristics Synonyms: Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, 2.7.11.24, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2
Immunogen Purified recombinant fragment of human MAPK expressed in E. Coli.

Antigen Type: Recombinant Protein
Clone 3F8
Isotype IgG2b

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN2869484) Images back to top
Antigen
Alternative Name MAPK1 (MAPK1 Antibody Abstract)
Background Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.
Molecular Weight 41390 Da
Gene ID 5594
NCBI Accession NP_620407
UniProt P28482
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN2869484) Images back to top
Application Notes The suggested dilution is: Western Bloting: = 1:500 - 2000. Flow cytometry: = 1:200 - 400.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN2869484) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-ERK2 Antibody (ABIN2869484)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Background publications

Lønne, Masoumi, Lennartsson, Larsson: "Protein kinase Cdelta supports survival of MDA-MB-231 breast cancer cells by suppressing the ERK1/2 pathway." in: The Journal of biological chemistry, Vol. 284, Issue 48, pp. 33456-65, 2009

Tabakoff, Saba, Printz, Flodman, Hodgkinson, Goldman, Koob, Richardson, Kechris, Bell, Hübner, Heinig, Pravenec, Mangion, Legault, Dongier, Conigrave, Whitfield, Saunders, Grant, Hoffman,: "Genetical genomic determinants of alcohol consumption in rats and humans." in: BMC biology, Vol. 7, pp. 70, 2009

Schiemann, Graves, Baumann, Morella, Gearing, Nielsen, Krebs, Nathanson: "Phosphorylation of the human leukemia inhibitory factor (LIF) receptor by mitogen-activated protein kinase and the regulation of LIF receptor function by heterologous receptor activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 12, pp. 5361-5, 1995

Gonzalez, Raden, Rigby, Davis: "Heterogeneous expression of four MAP kinase isoforms in human tissues." in: FEBS letters, Vol. 304, Issue 2-3, pp. 170-8, 1992