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anti-Guinea Pig HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus)
608
150
136
40
36
27
24
22
18
16
9
8
4
3
2
2
1
1
1
1
Host
Mouse
450
186
1
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
28
25
17
13
13
11
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
515
254
226
140
125
106
96
40
34
26
18
15
5
2
2
2
Supplier
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Catalog No. ABIN263953
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN361756 ICC IF IP IHC WB Mouse Log in to see 8A7 9
0.12303223 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
0.0011806369 ABIN2481427 ICC IF IP IHC WB Streptavidin Mouse Log in to see 8A7
0.0011806369 ABIN2481425 ICC IF IP IHC WB PerCP Mouse Log in to see 8A7
0.0011806369 ABIN2481413 ICC IF IP IHC WB Atto 565 Mouse Log in to see 8A7
0.0011806369 ABIN2481426 ICC IF IP IHC WB PE Mouse Log in to see 8A7
0.0011806369 ABIN2481419 ICC IF IP IHC WB Alkaline Phosphatase (AP) Mouse Log in to see 8A7
0.0011806369 ABIN2481411 ICC IF IP IHC WB Atto 390 Mouse Log in to see 8A7
0.0011806369 ABIN2481417 ICC IF IP IHC WB Atto 680 Mouse Log in to see 8A7
0.0011806369 ABIN2481420 ICC IF IP IHC WB APC Mouse Log in to see 8A7
0.0011806369 ABIN2481414 ICC IF IP IHC WB Atto 594 Mouse Log in to see 8A7
0.0011806369 ABIN2481418 ICC IF IP IHC WB Atto 700 Mouse Log in to see 8A7
0.0011806369 ABIN2481421 ICC IF IP IHC WB Biotin Mouse Log in to see 8A7
0.0011806369 ABIN2481416 ICC IF IP IHC WB Atto 655 Mouse Log in to see 8A7
0.0011806369 ABIN2481412 ICC IF IP IHC WB Atto 488 Mouse Log in to see 8A7
0.0011806369 ABIN2481422 ICC IF IP IHC WB FITC Mouse Log in to see 8A7
0.0011806369 ABIN2481423 ICC IF IP IHC WB HRP Mouse Log in to see 8A7
0.0011806369 ABIN2481424 ICC IF IP IHC WB PE-Atto 594 Mouse Log in to see 8A7
0.0011806369 ABIN2481415 ICC IF IP IHC WB Atto 633 Mouse Log in to see 8A7
0.0011806369 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 8A7

Top referenced anti-HSPB1 antibody for Western Blotting

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Dog (Canine) Mouse (Murine) Cow (Bovine) Hamster Guinea Pig
Western Blotting (WB) 498 Antibodies 105 Antibodies 34 Antibodies 112 Antibodies 36 Antibodies 24 Antibodies 27 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 120 Antibodies 28 Antibodies 29 Antibodies 28 Antibodies 24 Antibodies 23 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 108 Antibodies 26 Antibodies 27 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Rat (Rattus)
(608), (150), (136), (40), (36), (27), (24), (22), (18), (16), (9), (8), (4), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(450), (186), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(28), (25), (17), (13), (13), (11), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(515), (254), (226), (140), (125), (106), (96), (40), (34), (26), (18), (15), (5), (2), (2), (2)
Pubmed 8 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) Images
Specificity Detects a 25 kDa or 27 kDa protein corresponding to the Molecular Mass of Hsp25/27 on SDS PAGE immunoblots. Cross-reacts with Alpha B Crystallin.
Characteristics Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Purification Purified
Immunogen Hsp27 peptide
Clone 8A7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN263953) Images back to top
Application Notes Western blot (6): 1: 5000 (ECL). Immunoflourescence (6): 1: 200. Immunoprecipitation (9). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN263953) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-HSPB1 Antibody (ABIN263953)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Arrigo: "In search of the molecular mechanism by which small stress proteins counteract apoptosis during cellular differentiation." in: Journal of cellular biochemistry, Vol. 94, Issue 2, pp. 241-6, 2005

Van Montfort, Slingsby, Vierling: "Structure and function of the small heat shock protein/alpha-crystallin family of molecular chaperones." in: Advances in protein chemistry, Vol. 59, pp. 105-56, 2002

Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001

Sarto, Binz, Mocarelli: "Heat shock proteins in human cancer." in: Electrophoresis, Vol. 21, Issue 6, pp. 1218-26, 2000

Kim, Kim, Kim: "Crystal structure of a small heat-shock protein." in: Nature, Vol. 394, Issue 6693, pp. 595-9, 1998

Ehrnsperger, Gräber, Gaestel, Buchner: "Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation." in: The EMBO journal, Vol. 16, Issue 2, pp. 221-9, 1997

Ciocca, Oesterreich, Chamness, McGuire, Fuqua: "Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review." in: Journal of the National Cancer Institute, Vol. 85, Issue 19, pp. 1558-70, 1993

Welch: "Phorbol ester, calcium ionophore, or serum added to quiescent rat embryo fibroblast cells all result in the elevated phosphorylation of two 28,000-dalton mammalian stress proteins." in: The Journal of biological chemistry, Vol. 260, Issue 5, pp. 3058-62, 1985

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN263953) back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN263953) anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN263953) anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Image 2)