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anti-Human HSPB1 antibody for ELISA

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Rat (Rattus)
753
273
262
87
79
76
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Mouse
480
312
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
669
290
265
238
231
191
183
128
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3308015 ABIN516740 IF IP PLA ELISA WB Mouse IgG2a kappa AA 96-205, partial Log in to see 3G3
0.3308015 ABIN1845754 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.3308015 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
0.0012043115 ABIN361649 ICC IF IP IHC ELISA WB Mouse Log in to see 5D12-A12 7
0.0012043115 ABIN1531866 IHC ELISA WB Rabbit IgG pSer15 Log in to see Polyclonal
0.0012043115 ABIN1531867 ELISA WB Rabbit IgG pSer78 Log in to see Polyclonal
0.0012043115 ABIN1531868 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0012043115 ABIN933725 IHC ELISA WB Mouse IgG1 kappa AA 1-205 Log in to see 2A5 1
0.0012043115 ABIN165408 ELISA WB Mouse TBD, kappa chain Log in to see 2A5 1
0.0012043115 ABIN1532897 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012043115 ABIN1532898 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012043115 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012043115 ABIN100223 IP IHC ELISA WB Mouse IgG Log in to see G3-1
0.0012043115 ABIN1498733 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7
0.0012043115 ABIN2878840 IHC (p) ELISA WB Rabbit IgG AA 48-97 Log in to see Polyclonal
0.0012043115 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
0.0012043115 ABIN2888439 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0012043115 ABIN1996515 IP ELISA WB Mouse IgG2b AA 1-205 Log in to see 4E 8H3
0.0012043115 ABIN1102201 IHC (p) IP ELISA WB Mouse IgG2b, kappa Log in to see 5D12-A12
0.0012043115 ABIN364326 IHC (p) ELISA WB Mouse IgG1 AA 1-205 Log in to see 2A5

Top referenced anti-HSPB1 antibody for ELISA

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 43 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 15 Antibodies
Flow Cytometry (FACS) 102 Antibodies 124 Antibodies
Immunocytochemistry (ICC) 125 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 146 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 125 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 227 Antibodies
Dot Blot (DB) 18 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(753), (273), (262), (87), (79), (76), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(480), (312), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(669), (290), (265), (238), (231), (191), (183), (128), (68), (34), (18), (15), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-HSPB1 Antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...