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anti-Human HSPB1 antibody for Immunofluorescence

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
AA 80-150, Internal Region
78
64
51
45
23
17
16
16
15
12
12
9
6
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
806
280
275
87
78
72
71
51
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Rabbit
485
340
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
695
324
271
250
220
207
168
121
61
40
17
14
11
8
6
4
2
2
1
1
Supplier
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Catalog No. ABIN4319980
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.813655 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
11.813655 ABIN1107614 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5D7 2
11.813655 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
11.813655 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 8A7
11.813655 ABIN1870247 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal
11.813655 ABIN1870249 IF IHC WB Rabbit IgG pSer78 Log in to see Polyclonal
11.813655 ABIN1873103 IF WB Rabbit IgG Log in to see Polyclonal
11.813655 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
11.813655 ABIN1870246 IF IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
11.813655 ABIN267333 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
11.813655 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
11.813655 ABIN1996519 FACS IF IF/ICC Rabbit IgG AA 1-205 Log in to see 155
11.813655 ABIN4320010 ICC IF WB Chicken Log in to see Polyclonal
11.813655 ABIN2649210 FACS IF IF/ICC Mouse IgG1 AA 1-205 Log in to see 09
11.813655 ABIN516740 IF IP PLA ELISA WB Mouse IgG2a kappa AA 96-205, partial Log in to see 3G3
11.813655 ABIN2424911 IF IHC WB Rabbit IgG pSer15 Log in to see Polyclonal
11.813655 ABIN2425005 IF IHC WB Rabbit IgG pSer78 Log in to see Polyclonal
11.813655 ABIN2406882 IF IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
11.813655 ABIN3019893 IF IHC WB Rabbit pSer78 Log in to see Polyclonal
11.813655 ABIN3019755 IF IHC WB Rabbit pSer15 Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Immunofluorescence

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies
ELISA 59 Antibodies 55 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Flow Cytometry (FACS) 101 Antibodies 93 Antibodies
Immunocytochemistry (ICC) 102 Antibodies
Immunofluorescence (IF) 128 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies
Immunoprecipitation (IP) 59 Antibodies
Western Blotting (WB) 222 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 80-150, Internal Region
(78), (64), (51), (45), (23), (17), (16), (16), (15), (12), (12), (9), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(806), (280), (275), (87), (78), (72), (71), (51), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(485), (340), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(695), (324), (271), (250), (220), (207), (168), (121), (61), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN4319980) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot P04792]

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN4319980) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Gene Symbol: HSPB1
Gene ID 3315
UniProt P04792, P14602
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN4319980) Images back to top
Application Notes Western Blot 2 μg/mL, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100This HSP27 antibody is useful for ICC/IF, IHC-P, and Western blot where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN4319980) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-HSPB1 Antibody (ABIN4319980)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

López-González, Carmona, Arregui, Kovacs, Ferrer: "αB-crystallin and HSP27 in glial cells in tauopathies." in: Neuropathology : official journal of the Japanese Society of Neuropathology, Vol. 34, Issue 6, pp. 517-26, 2014 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Stice, Chen, Kim, Jung, Tran, Liu, Knowlton: "17β-Estradiol, aging, inflammation, and the stress response in the female heart." in: Endocrinology, Vol. 152, Issue 4, pp. 1589-98, 2011 (Sample species: Rat (Rattus)). Further details: Western Blotting

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN4319980) back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 80-150) (ABIN4319980) Western Blot: Hsp27 Antibody [NBP1-75477] - Analysis of HSP27 in human skeletal muscl...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 80-150) (ABIN4319980) Western Blot: Hsp27 Antibody [NBP1-75477] - analysis of Jurkat cell lysate (30ug) usi...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 80-150) (ABIN4319980) Immunocytochemistry/Immunofluorescence: Hsp27 Antibody [NBP1-75477] - HSP27 antibody ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 80-150) (ABIN4319980) Immunohistochemistry: Hsp27 Antibody [NBP1-75477] - Staining of HSP27 in mouse heart ...