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anti-Monkey HSPB1 antibody for Immunohistochemistry (Frozen Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Monkey
751
272
260
86
79
76
73
53
41
38
24
21
18
8
4
4
3
2
1
1
1
Host
Mouse
480
310
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
667
289
266
238
228
188
184
128
67
34
18
15
5
2
2
2
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Catalog No. ABIN187604
$ 684.20
Plus shipping costs $45.00

Similar anti-HSPB1 Antibodies

Application / Reactivity Monkey Human
ELISA 3 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 15 Antibodies
Flow Cytometry (FACS) 2 Antibodies 123 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 173 Antibodies
Immunoelectron Microscopy (IEM) 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 19 Antibodies
Dot Blot (DB) 19 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Monkey
(751), (272), (260), (86), (79), (76), (73), (53), (41), (38), (24), (21), (18), (8), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(480), (310), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(667), (289), (266), (238), (228), (188), (184), (128), (67), (34), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This antigen recognizes a 24-27 kDa estrogen-regulated protein known as heat shock protein 27 (HSP27). Positive Controls: BT474 and breast carcinoma cells. Species Reactivity: Human, monkey and chimpanzee, others not tested.
Immunogen Partially purified HSP27 protein from breast cancer MCF-7 cells.Produced by fusion between BALB/c splenocytes and mouse myeloma p3NS-1/Ag4-1 (NS1) cells.
Clone G3-1
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K proteins. About 50% of breast carcinomas are positive for Hsp27, especially those that are also positive for estrogen and or progesterone receptor. Hsp27 has also been implicated in drug resistance in cancer cells.
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Immunohistochemistry: Frozen and formalin/ paraffin- Use at 1-2 µg/mL for 60 min at RT Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. Western blotting: Use at 1-2 µg/mL for 2 hrs. at RT The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Buffer Clone G3.1 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100 µg/500 µL in 10 mM PBS, ph. 7.4, with protein stabilizer and 15 mM sodium azide. Purified from ascites fluid by Protein G chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C