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|Application / Reactivity||Monkey||Human|
|ELISA||4 Antibodies||257 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||15 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||123 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||171 Antibodies|
|Immunoelectron Microscopy (IEM)||1 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||4 Antibodies|
|Immunohistochemistry (IHC)||8 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||20 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN222927) Images
|Specificity||The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, dog (weakly) and pig (weakly) origins.|
|Immunogen||Recombinant human Hsp27|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN222927) Images back to top
|Alternative Name||Hsp27 (HSPB1 Antibody Abstract)|
Human Hsp27, mouse Hsp25 and αβ-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data sμggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
Synonyms: HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25
|Research Area||Cardiovascular, Contractility, Atherosclerosis, Heat Shock Proteins, Cytoskeleton, Microfilaments|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN222927) Images back to top
Western blotting (1-2 μg/mL), Immunoprecipitation (5-10 μg/mL), Immunocytochemistry (10-15 μg/mL), and ELISA (1 μg/mL).
However, the optimal conditions should be determined individually.
Country of Animal Origin: USA
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN222927) Images back to top
|Buffer||100 μg (200 μg/mL) in phosphate buffered saline (PBS), pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-HSPB1 Antibody (ABIN222927)Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
|Product cited in:||
Cheng, Pollock, Mahimkar, Olson, Lovett: "Matrix metalloproteinase 2 and basement membrane integrity: a unifying mechanism for progressive renal injury." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 11, pp. 1898-900, 2006