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anti-Mouse (Murine) HSPB1 antibody for ELISA

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
pSer82
67
65
52
40
25
22
16
16
13
13
12
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
753
273
261
87
79
78
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
480
313
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
670
291
265
240
231
190
185
128
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN1531868
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012117105 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN3185106 ELISA IHC WB Rabbit IgG Ser505 Log in to see Polyclonal
0.0012117105 ABIN1859221 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-209 Log in to see Polyclonal
0.0012117105 ABIN3182035 ELISA IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
0.0012117105 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012117105 ABIN1585689 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012117105 ABIN2622148 ELISA WB Biotin Rabbit IgG AA 1-209 Log in to see Polyclonal
0.0012117105 ABIN2471981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2622145 ELISA Rabbit IgG AA 1-209 Log in to see Polyclonal
0.0012117105 ABIN4320040 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320041 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320043 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320044 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320045 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320048 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320050 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM252
0.0012117105 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Western Blotting (WB) 638 Antibodies 226 Antibodies 231 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 165 Antibodies 82 Antibodies 83 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 218 Antibodies 124 Antibodies 126 Antibodies
Immunohistochemistry (IHC) 280 Antibodies 83 Antibodies 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 65 Antibodies 49 Antibodies 49 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer82
(67), (65), (52), (40), (25), (22), (16), (16), (13), (13), (12), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(753), (273), (261), (87), (79), (78), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(480), (313), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Western Blotting (WB)
(670), (291), (265), (240), (231), (190), (185), (128), (68), (34), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity HSP27 (Phospho-Ser82) Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser82.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Molecular weight of target protein: 22 kDa
Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth- related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27
Molecular Weight 22 kDa
Gene ID 3315
OMIM 602195
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.520973 (NCBI Gene Symbol: HSPB1)

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN1531868) Western blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 30', usin...