anti-Mouse (Murine) HSPB1 antibody for ELISA

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
pSer82
79
64
51
45
24
16
15
15
15
13
12
9
6
6
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
810
282
276
96
87
72
71
69
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Rabbit
489
340
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
ELISA, Western Blotting (WB)
699
326
270
252
221
208
168
157
61
40
17
11
11
8
6
4
2
2
1
1
1
Options
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Catalog No. ABIN1531868
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3185106 ELISA IHC WB Rabbit IgG Ser505 Log in to see Polyclonal
1 ABIN3182035 ELISA IHC WB Rabbit IgG pSer82 Log in to see Polyclonal
1 ABIN1532899 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2878840 IHC (p) ELISA WB Rabbit IgG AA 48-97 Log in to see Polyclonal
1 ABIN2888439 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
1 ABIN1735607 IHC (p) ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4320058 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320055 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320034 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320037 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320043 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320036 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320038 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320048 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320057 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320050 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320054 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320060 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320031 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see G3-1

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies 4 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies 8 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 677 Antibodies 223 Antibodies 227 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 159 Antibodies 59 Antibodies 63 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 250 Antibodies 114 Antibodies 115 Antibodies
Immunohistochemistry (IHC) 319 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer82
(79), (64), (51), (45), (24), (16), (15), (15), (15), (13), (12), (9), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(810), (282), (276), (96), (87), (72), (71), (69), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(489), (340), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application ELISA, Western Blotting (WB)
(699), (326), (270), (252), (221), (208), (168), (157), (61), (40), (17), (11), (11), (8), (6), (4), (2), (2), (1), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity HSP27 (Phospho-Ser82) Antibody detects endogenous levels of HSP27 only when phosphorylated at Ser82.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Molecular weight of target protein: 22 kDa
Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth- related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27
Molecular Weight 22 kDa
Gene ID 3315
OMIM 602195
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:4000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.520973 (NCBI Gene Symbol: HSPB1)

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN1531868) Western blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 30', usin...