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anti-Rat (Rattus) HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Human, Rat (Rattus)
806
281
275
87
78
72
71
51
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Mouse
486
339
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
695
324
270
251
221
207
168
120
61
40
17
14
11
8
6
4
2
2
1
1
Options
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.506455 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
13.506455 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
13.506455 ABIN1845754 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
1 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
1 ABIN1498733 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 5D7
1 ABIN3025020 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see HSPB1-774
1 ABIN3025018 FACS IF IHC WB Mouse IgG1, kappa Log in to see SPM252
1 ABIN3026467 FACS IF IHC WB Mouse IgG1, kappa Log in to see G3-1
1 ABIN4320030 FACS Allophycocyanin Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320058 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320057 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320035 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320055 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320067 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774

Top referenced anti-HSPB1 antibody for Flow Cytometry

  • Human Monoclonal HSPB1 Primary Antibody for FACS, IF - ABIN966308 (2 Publications): Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response. in Clinical cancer research : an official journal of the American Association for Cancer Research 2008 (PubMed)

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Human
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies
ELISA 59 Antibodies 259 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 101 Antibodies
Immunocytochemistry (ICC) 102 Antibodies
Immunofluorescence (IF) 128 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies
Immunoprecipitation (IP) 59 Antibodies
Western Blotting (WB) 222 Antibodies
Cell Culture (CC) 1 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA (Detection) 1 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(806), (281), (275), (87), (78), (72), (71), (51), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(486), (339), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(695), (324), (270), (251), (221), (207), (168), (120), (61), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-HSPB1 Antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...