anti-Rat (Rattus) HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Human, Rat (Rattus)
830
280
275
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Mouse
511
337
2
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
32
22
21
13
13
13
13
11
11
10
10
10
10
10
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
719
334
271
265
232
211
169
155
59
40
17
14
11
8
6
4
2
2
1
1
1
Options
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.327658 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
14.327658 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
14.327658 ABIN1845754 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
1 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1498733 ELISA FACS ICC IF IHC (p) WB Mouse IgG1 Log in to see 5D7
1 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
1 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320067 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320068 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
1 ABIN4320020 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320021 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320022 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320023 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320024 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320025 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320007 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
1 ABIN2481426 FACS ICC IF IHC IP WB PE Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481427 FACS ICC IF IHC IP WB Streptavidin Mouse IgG1 kappa Log in to see 8A7
1 ABIN2481411 FACS ICC IF IHC IP WB Atto 390 Mouse IgG1 kappa Log in to see 8A7

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(830), (280), (275), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(511), (337), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (32), (22), (21), (13), (13), (13), (13), (11), (11), (10), (10), (10), (10), (10), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(719), (334), (271), (265), (232), (211), (169), (155), (59), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. Coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 antibody (ABIN969199) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-HSPB1 antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...