We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)||Human|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies||8 Antibodies|
|ELISA||59 Antibodies||264 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||119 Antibodies|
|Immunocytochemistry (ICC)||102 Antibodies|
|Immunofluorescence (IF)||127 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||81 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||45 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||115 Antibodies|
|Immunoprecipitation (IP)||59 Antibodies|
|Western Blotting (WB)||222 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Dot Blot (DB)||17 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Epitope||AA 88-105, Middle Region Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.|
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: heat shock 27 kDa protein 1
Protein Name: Heat shock protein beta-1(HspB1)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Hsp27(88-105aa KHEERQDEHGYISRCFTR), identical to the related rat and mouse sequences.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 (HSPB1 Antibody Abstract)|
HSPB1(Heat shock 27 kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. Stock et al.(2003) used FISH to map the HSP27 gene to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F(CMT2F) and distal hereditary motor neuropathy(dHMN).
Synonyms: Heat shock 27 kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|Heat Shock Protein27 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|Hsp 27 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top