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anti-Rat (Rattus) HSPB1 antibody for Immunohistochemistry (Frozen Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
pSer82
78
63
51
45
24
17
16
16
15
13
12
9
6
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
809
282
276
87
78
73
71
51
46
32
30
27
19
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Mouse
487
341
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
698
327
272
251
224
211
170
121
60
40
17
14
11
8
6
4
2
2
1
1
Supplier
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Catalog No. ABIN126812
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126811 IHC (fro) WB Biotin Mouse IgG2a pSer82 Log in to see 5B9
1 ABIN3044184 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105, Middle Region Log in to see Polyclonal
1 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
1 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
1 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320058 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320057 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320035 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320055 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320054 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320060 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see G3-1
1 ABIN2622151 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see SJ-25
1 ABIN2622152 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105 Log in to see Polyclonal
1 ABIN4320024 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320031 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320034 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320025 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1

Similar anti-HSPB1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies 4 Antibodies 3 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies 8 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 676 Antibodies 223 Antibodies 47 Antibodies 227 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 161 Antibodies 60 Antibodies 64 Antibodies 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 249 Antibodies 114 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope pSer82
(78), (63), (51), (45), (24), (17), (16), (16), (15), (13), (12), (9), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(809), (282), (276), (87), (78), (73), (71), (51), (46), (32), (30), (27), (19), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(487), (341), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(698), (327), (272), (251), (224), (211), (170), (121), (60), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This antibody specifically interacts with HSP27 phosphorylated at Serine 82 and does not crossreact with the non-phosphorylated HSP27 nor with unrelated Serine-phosphorylated proteins.
Characteristics Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Purification Size Exclusion Chromatography
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjugated to KLH.
Clone 5B9
Isotype IgG2a

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from EGF-treated HepG2 cellsImmunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (pSer82) (ABIN126812) anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody