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Human HSPB1 ELISA Kit

Recommended HSPB1 Product (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kits
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
AA 2-205
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
32
11
10
5
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN921115
$ 380.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.3112877 ABIN414602 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.78-50 ng/mL 0.78 ng/mL Log in to see
0.3112877 ABIN2708242 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.3112877 ABIN2114118 Cell Culture Supernatant, Cell Lysate, Serum, Plasma (EDTA - heparin), Tissue Homogenate Log in to see
0.0012097829 ABIN626481 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012097829 ABIN3199221 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012097829 ABIN1380605 Cell ELISA Log in to see
0.0012097829 ABIN1380607 Cell ELISA Log in to see
0.0012097829 ABIN1380606 Cell ELISA Log in to see
0.0012097829 ABIN1570401 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.781-50 ng/mL 0.781 ng/mL Log in to see
0.0012097829 ABIN1112620 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012097829 ABIN1381218 Cell ELISA Log in to see
0.0012097829 ABIN455298 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012097829 ABIN625460 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 0.15-50 ng/mL 0.15 ng/mL Log in to see
0.0012097829 ABIN2748167 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids Log in to see
0.0012097829 ABIN2064706 Cell ELISA Log in to see
0.0012097829 ABIN2064709 Cell ELISA Log in to see
0.0012097829 ABIN2064711 Cell ELISA Log in to see
0.0012097829 ABIN2684517 Sandwich ELISA Cell Lysate, Plasma, Serum, Tissue Samples 0.39-25 ng/mL 0.39 ng/mL Log in to see
0.0012097829 ABIN2684518 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012097829 ABIN2684519 Sandwich ELISA Media, Cell Lysate, Serum 94-6000 pg/mL 94 pg/mL Log in to see

Top referenced HSPB1 ELISA Kit for ELISA

Similar HSPB1 ELISA Kits

Application / Reactivity Human
ELISA 33 ELISA Kits

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kits
Epitope AA 2-205
Reactivity Human
Kits with alternative reactivity to:
(32), (11), (10), (5), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details HSPB1 ELISA Kit

Target details Application Details Handling References for HSPB1 Kit (ABIN921115) Images
Purpose For quantitative detection of human HSP27 in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant human HSP27
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- human HSP27 antibod - 1
  • Lyophilized recombinant human HSP27 standar - 10ng/tubex2
  • Biotinylated anti- human HSP27 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: T2-K205

Target details

Product Details HSPB1 ELISA Kit Application Details Handling References for HSPB1 Kit (ABIN921115) Images back to top
Antigen
Alternative Name HSPB1 (HSPB1 ELISA Kit Abstract)
Background Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7 kDa.
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details HSPB1 ELISA Kit Target details Handling References for HSPB1 Kit (ABIN921115) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human HSP27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HSP27 has been precoated onto 96-well plates. Standards(E.coli, T2-K205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HSP27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HSP27 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human HSP27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HSP27 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 256, Standard deviation: 10.75, CV(%): 4.2
  • Sample 2: n=16, Mean(pg/ml): 1369, Standard deviation: 73.9, CV(%): 5.4
  • Sample 3: n=16, Mean(pg/ml): 2628, Standard deviation: 144.5, CV(%): 5.5,
  • Sample 1: n=24, Mean(pg/ml): 279, Standard deviation: 17.6, CV(%): 6.3
  • Sample 2: n=24, Mean(pg/ml): 1406, Standard deviation: 105.45, CV(%): 7.5
  • Sample 3: n=24, Mean(pg/ml): 2635, Standard deviation: 213.4, CV(%): 8.1
Restrictions For Research Use only

Handling

Product Details HSPB1 ELISA Kit Target details Application Details References for HSPB1 Kit (ABIN921115) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for HSPB1 Kit (ABIN921115)

Product Details HSPB1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Hunt, Goswami, Kozak: "Assignment of the mouse Hsp25 and Hsp105 genes to the distal region of chromosome 5 by linkage analysis." in: Genomics, Vol. 45, Issue 2, pp. 462-3, 1998

Images

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Supplier Images
ELISA image for HSPB1 ELISA Kit (Heat Shock 27kDa Protein 1) (ABIN921115) Human HSP27 PicoKine ELISA Kit standard curve