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anti-Rat (Rattus) JNK antibody for Functional Studies

Recommended JNK Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
  • BSK
  • BSK/DJNK
  • Bsk
  • CG5680
  • D-JNK
  • D-junk
  • DBSK/JNK
  • DJNK
  • DJNK/bsk
  • Dmel\\CG5680
  • JNK
  • JNK/SAPK
  • Jnk
  • Junk
  • SAPKa
  • c-Jun
  • dJNK
  • jnk
  • JNK-46
  • JNK1
  • JNK1A2
  • JNK21B1/2
  • PRKM8
  • SAPK1
  • SAPK1c
  • AI849689
  • Prkm8
  • jnk1
  • sapk1
  • T10F20.15
  • mapk8
  • zgc:112379
  • basket
  • mitogen-activated protein kinase 8
  • mitogen-activated protein kinase 8b
  • Protein JNK-1
  • bsk
  • MAPK8
  • Mapk8
  • mapk8
  • ATMPK8
  • mapk8b
  • jnk-1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
378
177
147
23
19
13
8
6
5
4
3
3
3
2
2
2
2
1
1
Host
Rabbit
300
96
2
Clonality
Polyclonal
Conjugate
This JNK antibody is un-conjugated
18
16
14
11
11
10
4
3
3
3
3
3
3
3
3
3
3
Application
Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
303
191
92
73
57
55
39
37
17
16
12
8
4
3
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN210955
$ 771.83
Plus shipping costs $45.00

Similar anti-JNK Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 33 Antibodies 47 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Flow Cytometry (FACS) 8 Antibodies 12 Antibodies
Functional Studies (Func) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(378), (177), (147), (23), (19), (13), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(300), (96), (2)
Clonality
Polyclonal
Conjugate This JNK antibody is un-conjugated
(18), (16), (14), (11), (11), (10), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(303), (191), (92), (73), (57), (55), (39), (37), (17), (16), (12), (8), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-JNK Antibody

Target Details JNK Application Details Handling Images
Specificity Recognizes SAPK1a (54kD). Weakly recognizes SAPK1b (54kD). The protein detected with a MWapp of 45kD may be SAPK1c. Species Reactivity: Human, mouse and rat.
Purification Protein A purified
Immunogen GST fusion protein corresponding to full-length rat SAPK1a. This sequence is approximately 85% identical to rat SAPK1b and rat SAPK1c. These sequences are highly conserved among mammalian species.

Type of Immunogen: Fusion protein
Isotype IgG

Target Details JNK

Product Details anti-JNK Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPK8 / JNK1 / JNK (MAPK8 Antibody Abstract)
Background Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase
Gene ID 5599
UniProt P45983
Pathways MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-JNK Antibody Target Details JNK Handling Images back to top
Application Notes Approved: Func, IP, WB

Usage: Suitable for use in Western Blot analysis and Immunoprecipitation. Western Blot Analysis: 1 μg/mL detected SAPK1 in RIPA lysates from human A431, murine 3T3 and rat PC-12 cells. At 1 μg/mL, the signal detected with 10 ng of gst-SAPK1a was approximately three times greater than the signal detected of with 10 ng gst-SAPK1b. Included Positive Antigen Control: Non-stimulated A431 cell lysate. Use 20 μg per lane for minigels. Immunoprecipitation Protein Kinase Assay: 4 μg immunoprecipitated active recombinant rat SAPK1a, which then catalyzed the transfer of the y-phosphate of [y-32 P]-ATP to c-Jun protein substrate. Western Blot Analysis A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SAPK1 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Restrictions For Research Use only

Handling

Product Details anti-JNK Antibody Target Details JNK Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.01 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.