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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||29 Antibodies||37 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies||8 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||19 Antibodies||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Immunohistochemistry (IHC)||26 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||74 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 8 (MAPK8) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This JNK antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-JNK AntibodyTarget Details JNK Application Details Handling Images
|Specificity||This antibody detects endogenous levels of p-JNK1/2/3 protein only when phosphorylated at Thr183.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details JNKProduct Details anti-JNK Antibody Application Details Handling Images back to top
|Alternative Name||MAPK8 / JNK1 (MAPK8 Antibody Abstract)|
|Background||JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun Nterminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.Synonyms: JNK-1, JNK-46, JNK2, JNK3, MAPK8, Mitogen-activated protein kinase 8, PRKM8, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1|
|Molecular Weight||approx. 46,54 kDa|
|Pathways||MAPK Signaling, WNT Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-JNK Antibody Target Details JNK Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-JNK Antibody Target Details JNK Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-JNK Antibody Target Details JNK Application Details Handling back to top