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|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Epitope||pSer218, pSer222, pSer226 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling Images
|Specificity||AM00090PU-N specifically recognizes MEK1 phosphorylated at serine 218/222 and MEK2 phosphorylated at serine 222/226.|
|Characteristics||Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1,Dual specificity mitogen-activated protein kinase kinase 1|
|Purification||Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.|
|Components||incl. positive Control|
|Immunogen||Phosphopeptide conjugated to KLH. Epitope: pSer 218/222 in MEK1 pSer 222/226 in MEK2AA Sequence: D pS M A N pS FRemarks: Mol. Weight of Antigen: 45 kDa|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase 1 and 2 on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Suitable for Western Blot (0.5 μg/mL for HRPO/ECL detection)Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use SW480 pervanadate-treated as positive control (see Protocols below). ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Contains Positive Control (pervanadate treated SW 480 cells)Format: Lyophilized cell lysate from SW480 cells. Serum starved cells were treated for15min with pervanadate. Reconstitution: Add 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRP/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation. Aliquot and store frozen. Avoid repeated freeze/thaw cycles.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
|Reconstitution||Reconstitute with 1 mL H2O (15 min, RT)|
|Buffer||1 x PBS / 0.09 % Na-azide / PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.|
|Expiry Date||12 months|
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top