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anti-Rat (Rattus) MAP2K1 antibody for Enzyme Immunoassay

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
603
415
365
107
80
45
30
26
22
21
10
8
5
5
4
4
2
2
2
1
1
Host
Mouse
486
123
2
2
1
Clonality
Monoclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
6
6
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
466
195
150
121
120
78
25
22
16
10
9
6
6
5
2
2
2
1
1
1
Supplier
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Catalog No. ABIN126837
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126835 EIA WB Mouse IgG1 Log in to see 10B1

Similar anti-MAP2K1 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 1 Antibodies 3 Antibodies 2 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies
Western Blotting (WB) 456 Antibodies 308 Antibodies 277 Antibodies 103 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies 1 Antibodies 1 Antibodies
Luminex Assay (LMNX) 10 Antibodies
Intracellular Staining (ICS) 2 Antibodies 9 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(603), (415), (365), (107), (80), (45), (30), (26), (22), (21), (10), (8), (5), (5), (4), (4), (2), (2), (2), (1), (1)
Host Mouse
(486), (123), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(466), (195), (150), (121), (120), (78), (25), (22), (16), (10), (9), (6), (6), (5), (2), (2), (2), (1), (1), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Specificity This antibody specifically recognizes the activation loop of MEK1/2 independent of its phosphorylation status.
Characteristics Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1,MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2
Purification Size exclusion chromatography
Components incl. positive Control
Immunogen Synthetic peptide conjugated to KLH
Clone 9G3
Isotype IgG2a

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK1, MAPKK2, MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2
Gene ID 5604
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL (protein ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT)
Buffer 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (ABIN126837) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibody