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anti-Rat (Rattus) MAP2K1 antibody for Immunocytochemistry

Recommended MAP2K1 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
  • ATMEK1
  • CFC3
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MAPKK1
  • MEK
  • mek-2
  • MEK1
  • Mek1
  • MEKK1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • MKK1
  • NMAPKK
  • PRKMK1
  • Prkmk1
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
Epitope
AA 353-367, C-Term
43
38
35
32
32
31
30
27
25
22
21
15
15
13
13
13
11
9
7
7
6
6
6
6
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
606
324
280
60
38
24
15
14
13
6
4
4
4
2
2
Host
Rabbit
494
123
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
14
14
14
11
9
8
8
8
6
6
6
6
6
6
6
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
454
208
157
116
94
66
23
22
13
10
9
6
6
2
2
2
2
2
1
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Catalog No. ABIN3044391
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN966512 ICC IHC (p) WB C-Term, AA 353-367 Log in to see Polyclonal 2
0.0009962841 ABIN2448577 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009962841 ABIN4333482 ICC IF IHC IHC (p) WB Rabbit pThr292 Log in to see Polyclonal
0.0009962841 ABIN2987553 ICC IF IHC WB Rabbit IgG pSer221 Log in to see Polyclonal
0.0009962841 ABIN4333486 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN3031839 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-MAP2K1 antibody for Immunocytochemistry

Similar anti-MAP2K1 Antibodies

Application / Reactivity Rat (Rattus) Human
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 45 Antibodies 154 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 15 Antibodies
Immunofluorescence (IF) 50 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 111 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 206 Antibodies
Dot Blot (DB) 23 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies
Epitope AA 353-367, C-Term
(43), (38), (35), (32), (32), (31), (30), (27), (25), (22), (21), (15), (15), (13), (13), (13), (11), (9), (7), (7), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(606), (324), (280), (60), (38), (24), (15), (14), (13), (6), (4), (4), (4), (2), (2)
Host Rabbit
(494), (123), (2), (2), (1)
Clonality
Polyclonal
Conjugate This MAP2K1 antibody is un-conjugated
(14), (14), (14), (11), (9), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(454), (208), (157), (116), (94), (66), (23), (22), (13), (10), (9), (6), (6), (2), (2), (2), (2), (2), (1)
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Product Details anti-MAP2K1 Antibody

Target Details MAP2K1 Application Details Handling Images
Sequence KQLMVHAFIK RSDAE
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 1(MAP2K1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 1
Protein Name: Dual specificity mitogen-activated protein kinase kinase 1(MAP kinase kinase 1/)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1(353-367aa KQLMVHAFIKRSDAE), identical to the related mouse and rat sequences.
Isotype IgG

Target Details MAP2K1

Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (AA 353-367) (ABIN3044391) Anti-MEK1 antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (AA 353-367) (ABIN3044391) Anti-MEK1 antibody, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2...
Immunohistochemistry (IHC) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (AA 353-367) (ABIN3044391) Anti-MEK1 antibody, ICC ICC: MCF-7 Cell
Immunohistochemistry (IHC) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (AA 353-367) (ABIN3044391) Anti-MEK1 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue