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|Application / Reactivity||Rat (Rattus)||Human|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||45 Antibodies||154 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||6 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunofluorescence (IF)||50 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||55 Antibodies|
|Immunohistochemistry (IHC)||110 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||43 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||205 Antibodies|
|Dot Blot (DB)||23 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) Antibodies|
|Epitope||AA 353-367, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This MAP2K1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-MAP2K1 AntibodyTarget Details MAP2K1 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 1(MAP2K1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 1
Protein Name: Dual specificity mitogen-activated protein kinase kinase 1(MAP kinase kinase 1/)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1(353-367aa KQLMVHAFIKRSDAE), identical to the related mouse and rat sequences.|
Target Details MAP2K1Product Details anti-MAP2K1 Antibody Application Details Handling Images back to top
|Alternative Name||MAP2K1 (MAP2K1 Antibody Abstract)|
|Background||Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.|
|Research Area||Kinases/Phosphatases, Phospho-specific antibodies|
|Pathways||MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-MAP2K1 Antibody Target Details MAP2K1 Application Details Handling back to top