You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human MAP2K2 antibody for Immunofluorescence (Cultured Cells)

Recommended MAP2K2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
  • AA589381
  • ATMKK2
  • CFC4
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MAPKK2
  • MEK2
  • MK1
  • MK2
  • MKK2
  • PRKMK2
  • Prkmk2
Epitope
AA 1-50
33
23
15
14
14
14
12
10
10
8
8
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
230
106
89
8
4
3
2
2
Host
Rabbit
189
38
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
80
79
39
26
26
22
15
13
10
7
4
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN726500
$ 269.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008772887 ABIN906102 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN906104 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN906105 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN906106 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN726503 IF (cc) IF (p) Cy3 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN726504 IF (cc) IF (p) Cy5 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN726506 IF (cc) IF (p) Cy7 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN726505 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN726507 IF (cc) IF (p) FITC Rabbit IgG AA 1-50 Log in to see Polyclonal
0.0008772887 ABIN2808137 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 1-50 Log in to see Polyclonal

Similar anti-MAP2K2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 14 Antibodies 18 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 16 Antibodies 18 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Western Blotting (WB) 69 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies
Epitope AA 1-50
(33), (23), (15), (14), (14), (14), (12), (10), (10), (8), (8), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(230), (106), (89), (8), (4), (3), (2), (2)
Host Rabbit
(189), (38), (9), (1)
Clonality
Polyclonal
Conjugate This MAP2K2 antibody is un-conjugated
(10), (10), (9), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(187), (80), (79), (39), (26), (26), (22), (15), (13), (10), (7), (4), (2), (1), (1)
Supplier Log in to see

Product Details anti-MAP2K2 Antibody

Target Details MAP2K2 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human MAPKK2/MEK2
Isotype IgG

Target Details MAP2K2

Product Details anti-MAP2K2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MEK2 (MAP2K2 Antibody Abstract)
Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).

Subcellular location: Intracellular


Synonyms: CFC4, MEK2, MKK2, MAPKK2, PRKMK2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2
Gene ID 5605
UniProt P36507
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-50) (ABIN726500) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2/MAPK...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (AA 1-50) (ABIN726500) HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250....
'Independent Validation' Badge
Lot Number 110511
Method validated Western Blotting
Positive Control Hela cell extract
Negative Control c6/36 Mosquito cell extract (non-reactive species)
Notes A single positive band at the correct molecular weight was detected in positive control HeLa cell extract. Several bands were observed in the non-reactive species negative control at approximately the same molecular weight as MAP2K2. These bands may constitute cross-reactivity of the target antigen, or they may be non-specific bands.
Primary Antibody
  • Antigen: Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (1:150 dilution)
  • Catalog number: ABIN726500
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 110511
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: HeLa cell extract
  • Negative control: c6/36 Mosquito cell extract
Protocol
  • 1. Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 2. 46 μg of boiled extracts were loaded and resolved on 8-16% SDS-polyacrylamide gel.
  • 3. The Thermo Scientific - Spectra Multicolor Broad Range (Cat # 26634) were used as molecular mass markers.
  • 4. Proteins were then transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • 5. The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • 6. The membrane was rinsed with TBST once.
  • 7. The membrane was immersed with the protein side up in the primary antibody solution (anti-MAP2K2; 1:150) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 h at 4°C.
  • 8. The membrane was rinsed in TBST thrice for 5 min each.
  • 9. The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 h at room temperature (~26°C) with gentle agitation.
  • 10. The membrane was rinsed thrice TBST thrice for 5 min each.
  • 11. The membrane was rinsed in TBS twice for 30 s each.
  • 12. Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • 13. The membrane was rinsed three times TBST.
  • 14. Incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • 15. The membrane was washed in TBST 2 times for 10 min each.
  • 16. Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 1-50) antibody (ABIN726500) Figure 1. Western blot of lysates from HeLa cells (Lane 1) and c6/36 cells (Lane 2) p...