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|Antigen||Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) Antibodies|
|Epitope||AA 1-110 Alternatives|
|Reactivity||Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAP2K2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-MAP2K2 AntibodyTarget Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN967846) Images
|Cross-Reactivity||Mouse (Murine), Chicken, Dog (Canine), Frog, Human|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Rat MEK2 aa. 1-110|
Target Details MAP2K2Product Details anti-MAP2K2 Antibody Application Details Handling References for anti-MAP2K2 antibody (ABIN967846) Images back to top
|Alternative Name||MEK2 (MAP2K2 Antibody Abstract)|
MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase
|Molecular Weight||46 kDa|
|Research Area||Cancer, Tyrosine Kinases, Kinases/Phosphatases|
|Pathways||MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-MAP2K2 Antibody Target Details MAP2K2 Handling References for anti-MAP2K2 antibody (ABIN967846) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details References for anti-MAP2K2 antibody (ABIN967846) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-MAP2K2 antibody (ABIN967846)Product Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling Images back to top
|Product cited in:||
Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, 2002
Downey, Butler, Tapper, Fialkow, Saltiel, Rubin, Grinstein: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, 1998
Wu, Harrison, Dent, Lynch, Weber, Sturgill: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, 1993
Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992
Hattori, Fukuda, Yamashita, Nakamura, Gotoh, Nishida: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, 1992
ImagesProduct Details anti-MAP2K2 Antibody Target Details MAP2K2 Application Details Handling References for anti-MAP2K2 antibody (ABIN967846) back to top