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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)||Cow (Bovine)||Chicken||Zebrafish (Danio rerio)||Non Human Primates|
|Immunofluorescence (fixed cells) (IF/ICC)||8 Antibodies||4 Antibodies||4 Antibodies|
|Western Blotting (WB)||18 Antibodies||164 Antibodies||75 Antibodies||67 Antibodies||8 Antibodies||6 Antibodies||4 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||22 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies|
|Epitope||pThr180, pTyr182 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This MAPK14 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-MAPK14 AntibodyTarget Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN372694) Images
|Specificity||This antibody recognizes the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.|
Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Human.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK.|
Target Details MAPK14Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 Antibody (ABIN372694) Images back to top
|Alternative Name||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)|
|Background||The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002, Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003, Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha|
|Pathways||MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 Antibody (ABIN372694) Images back to top
Western blot: 1/1000. Immunohistochemistry: 1/250-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 Antibody (ABIN372694) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-MAPK14 Antibody (ABIN372694)Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Philip, Armstead: "Differential role of PTK, ERK and p38 MAPK in superoxide impairment of NMDA cerebrovasodilation." in: Brain research, Vol. 979, Issue 1-2, pp. 98-103, 2003
Ying, Futter, Rosenblum, Webber, Hunt, Bliss, Bramham: "Brain-derived neurotrophic factor induces long-term potentiation in intact adult hippocampus: requirement for ERK activation coupled to CREB and upregulation of Arc synthesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 5, pp. 1532-40, 2002
Shi, Gaestel: "In the cellular garden of forking paths: how p38 MAPKs signal for downstream assistance." in: Biological chemistry, Vol. 383, Issue 10, pp. 1519-36, 2002
Johnson, Lapadat: "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases." in: Science (New York, N.Y.), Vol. 298, Issue 5600, pp. 1911-2, 2002
Lin, Minden, Martinetto, Claret, Lange-Carter, Mercurio, Johnson, Karin: "Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2." in: Science (New York, N.Y.), Vol. 268, Issue 5208, pp. 286-90, 1995
ImagesProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN372694) back to top