MAPK14 antibody (pThr180)

Catalog No. ABIN7206474

The Rabbit Polyclonal anti-MAPK14 antibody is suitable to detect MAPK14 in samples from Human, Mouse, Rat and Guinea Pig. It has been validated for WB, IHC, ELISA, IF and FACS.

Quick Overview for MAPK14 antibody (pThr180) (ABIN7206474)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MAPK14 Antibody

Binding Specificity: pThr180

Purpose: Rabbit Anti-p38 (phospho Thr180/Y182) Polyclonal Antibody

Specificity: Phospho-p38 (T180/Y182) Polyclonal Antibody detects endogenous levels of p38 protein only when phosphorylated at T180/Y182.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human p38 Phospho-Thr180/Y182

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF/ICC1:100-500,WB 1:500-2000,Flow Cyt 1:50-200,IHC 1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: p38

Background: MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting proteinMitogen-activated protein kinase 14 encoded by MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Mitogen-activated protein kinase 14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with mitogen-activated protein kinase 14.The substrates of mitogen-activated protein kinase 14 include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of mitogen-activated protein kinase 14 in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of MAPK14 encoding distinct isoforms have been reported.

Molecular Weight: 38kD

Gene ID: 1432

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins