|Application / Reactivity||Dog (Canine)||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)||Chicken||Guinea Pig||Hamster||Monkey||Pig (Porcine)||Rabbit||Sheep (Ovine)|
|Western Blotting (WB)||19 Antibodies||164 Antibodies||77 Antibodies||72 Antibodies||9 Antibodies||7 Antibodies||3 Antibodies||3 Antibodies||8 Antibodies||3 Antibodies||3 Antibodies||3 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 14 (MAPK14) Antibodies|
|Epitope||AA 341-360 Alternatives|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This MAPK14 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-MAPK14 AntibodyTarget Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) Images
|Specificity||Detects ~43 kDa corresponding to the molecular mass of p38 on SDS-PAGE immunoblots.|
|Immunogen||A 20 residue synthetic peptide based on the Human p38 (residues 341-360) with the Cysteine residue added and coupled to KLH|
Target Details MAPK14Product Details anti-MAPK14 Antibody Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) Images back to top
|Alternative Name||MAP Kinase p38 alpha / MAPK14 (MAPK14 Antibody Abstract)|
|Background||The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha|
|Pathways||MAPK Signaling, TCR Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK14 Antibody Target Details MAPK14 Handling References for anti-MAPK14 Antibody (ABIN264893) Images back to top
Western blot: A 1/5000 dilution of this antibody was sufficient for detection of p38 in 20 μgof HeLa cell lysate by ECL Immunoblot analysis. Immunoprecipitation: 1/250. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details References for anti-MAPK14 Antibody (ABIN264893) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-MAPK14 Antibody (ABIN264893)Product Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling Images back to top
Fan, Chen, Qiao et al.: "Opposing effects of ERK and p38 MAP kinases on HeLa cell apoptosis induced by dipyrithione." in: Molecules and cells, Vol. 23, Issue 1, pp. 30-8, 2007 (PubMed).
Medicherla, Protter, Ma et al.: "Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes." in: The Journal of pharmacology and experimental therapeutics, Vol. 318, Issue 1, pp. 99-107, 2006 (PubMed).
Salojin, Owusu, Millerchip et al.: "Essential role of MAPK phosphatase-1 in the negative control of innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1899-907, 2006 (PubMed).
Van Laethem, Van Kelst, Lippens et al.: "Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 15, pp. 1946-8, 2004 (PubMed).
Deng, Ren, Yang et al.: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).
Pearson, Robinson, Beers Gibson et al.: "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions." in: Endocrine reviews, Vol. 22, Issue 2, pp. 153-83, 2001 (PubMed).
Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).
ImagesProduct Details anti-MAPK14 Antibody Target Details MAPK14 Application Details Handling References for anti-MAPK14 Antibody (ABIN264893) back to top