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anti-Human MAPK6 antibody for Flow Cytometry

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • 2610021I23Rik
  • ATMAPK6
  • ATMPK6
  • D130053K17Rik
  • Erk3
  • ERK3
  • ERK6
  • F18O19.10
  • HsT17250
  • Mapk4
  • MAPK6
  • Mapk63
  • MAP KINASE 6
  • MAP kinase 6
  • P38GAMMA
  • p97MAPK
  • Prkm4
  • Prkm6
  • PRKM6
  • PRKM12
  • SAPK-3
  • SAPK3
  • SUSERK3
Reactivity
Human
114
57
47
4
3
2
2
2
1
1
1
Host
Mouse
87
22
12
2
Clonality
Monoclonal
Conjugate
This MAPK6 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
88
67
34
24
11
9
7
6
5
2
1
1
1
Supplier
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Catalog No. ABIN969115
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30379575 ABIN1845601 FACS ELISA WB Mouse IgG1 Log in to see
0.0010485112 ABIN1499313 FACS ELISA WB Mouse IgG1 Log in to see 4E8
0.0010485112 ABIN966092 FACS ELISA WB Mouse IgG1 Log in to see 4E8 2
0.0010485112 ABIN865445 FACS ELISA WB Mouse IgG1 Log in to see 4E8
0.0010485112 ABIN2261954 FACS IHC WB Rabbit IgG N-Term Log in to see 13A179
0.0010485112 ABIN2261960 FACS ELISA WB Mouse IgG1 Log in to see 13B271
0.0010485112 ABIN4309449 ELISA FACS WB Mouse IgG1 Log in to see 4-00E08

Top referenced anti-MAPK6 antibody for Flow Cytometry

Similar anti-MAPK6 Antibodies

Application / Reactivity Human
ELISA 60 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 4 Antibodies
RNA Interference (RNAi) 2 Antibodies
Western Blotting (WB) 82 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Reactivity Human
(114), (57), (47), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(87), (22), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK6 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(88), (67), (34), (24), (11), (9), (7), (6), (5), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN969115) Images
Purification Ascites
Immunogen Purified recombinant fragment of human ERK3 expressed in E. coli.
Clone 4-00E-08
Isotype IgG1

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling References for anti-MAPK6 Antibody (ABIN969115) Images back to top
Antigen
Alternative Name ERK3 (MAPK6 Antibody Abstract)
Background The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. (provided by RefSeq) Tissue specificity: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts
Molecular Weight 105 kDa
Gene ID 5597
HGNC 5597
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling References for anti-MAPK6 Antibody (ABIN969115) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details References for anti-MAPK6 Antibody (ABIN969115) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-MAPK6 Antibody (ABIN969115)

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling Images back to top
Product cited in:

Klinger, Turgeon, Lévesque, Wood, Aagaard-Tillery, Meloche: "Loss of Erk3 function in mice leads to intrauterine growth restriction, pulmonary immaturity, and neonatal lethality." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 39, pp. 16710-5, 2009

Déléris, Rousseau, Coulombe, Rodier, Tanguay, Meloche: "Activation loop phosphorylation of the atypical MAP kinases ERK3 and ERK4 is required for binding, activation and cytoplasmic relocalization of MK5." in: Journal of cellular physiology, Vol. 217, Issue 3, pp. 778-88, 2008

Images

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN969115) back to top
Supplier Images
ELISA image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (ABIN969115) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (ABIN969115) Figure 2: Flow cytometric analysis of Hela cells using ERK3 mouse mAb (green) and neg...
Western Blotting (WB) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (ABIN969115) Figure 3: Western blot analysis using ERK3 mAb against HEK293 (1) and ERK3 (AA: 347-5...