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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)|
|Western Blotting (WB)||83 Antibodies||35 Antibodies||44 Antibodies|
|RNA Interference (RNAi)||2 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||4 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||6 Antibodies||5 Antibodies|
|Immunohistochemistry (IHC)||36 Antibodies||14 Antibodies||13 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||23 Antibodies||8 Antibodies||8 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies|
|Epitope||AA 520-721 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This MAPK6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-MAPK6 AntibodyTarget Details MAPK6 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase 6(MAPK6) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase 6
Protein Name: Mitogen-activated protein kinase 6
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human MAPK6 recombinant protein (Position: E520-N721). Human MAPK6 shares 86.2% and 82.8% amino acid (aa) sequence identity with mouse and rat MAPK6, respectively.|
Target Details MAPK6Product Details anti-MAPK6 Antibody Application Details Handling Images back to top
|Alternative Name||MAPK6 (MAPK6 Antibody Abstract)|
|Background||Mitogen-activated protein kinase 6 is an enzyme that in humans is encoded by the MAPK6 gene. It is mapped to 15q21. The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblastsupon treatment with serum or phorbol esters.|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MAPK6 Antibody Target Details MAPK6 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling back to top