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anti-Rat (Rattus) MAPK6 antibody for BioImaging

Recommended MAPK6 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
  • 2610021I23Rik
  • ATMAPK6
  • ATMPK6
  • D130053K17Rik
  • Erk3
  • ERK3
  • ERK6
  • F18O19.10
  • HsT17250
  • Mapk4
  • MAPK6
  • Mapk63
  • MAP KINASE 6
  • MAP kinase 6
  • P38GAMMA
  • p97MAPK
  • Prkm4
  • Prkm6
  • PRKM6
  • PRKM12
  • SAPK-3
  • SAPK3
  • SUSERK3
Epitope
AA 399-543
26
10
8
8
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
115
57
46
4
3
2
2
2
1
1
1
Host
Mouse
87
22
12
2
Clonality
Monoclonal
Conjugate
This MAPK6 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
88
68
34
23
11
9
8
5
5
2
1
1
Supplier
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Catalog No. ABIN967749
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Top referenced anti-MAPK6 antibody for BioImaging

Similar anti-MAPK6 Antibodies

Application / Reactivity Rat (Rattus)
BioImaging (BI) 1 Antibodies
ELISA 20 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 6 (MAPK6) Antibodies
Epitope AA 399-543
(26), (10), (8), (8), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(115), (57), (46), (4), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(87), (22), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPK6 antibody is un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application BioImaging (BI), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(88), (68), (34), (23), (11), (9), (8), (5), (5), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-MAPK6 Antibody

Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN967749) Images
Cross-Reactivity Mouse (Murine), Human, Chicken, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ERK3
Clone OPG-13
Isotype IgM

Target Details MAPK6

Product Details anti-MAPK6 Antibody Application Details Handling References for anti-MAPK6 Antibody (ABIN967749) Images back to top
Antigen
Alternative Name ERK3 (MAPK6 Antibody Abstract)
Background The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK3 is a 63kDa family member that is 43% homologous to ERK1 and ERK2. A 97kDa ERK3 isoform has also been described which possesses an additional 178 amino acids. This antibody is routinely tested by Western blot analysis and immunofluorescent imaging.
Molecular Weight 62 kDa
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPK6 Antibody Target Details MAPK6 Handling References for anti-MAPK6 Antibody (ABIN967749) Images back to top
Application Notes Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details References for anti-MAPK6 Antibody (ABIN967749) Images back to top
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-MAPK6 Antibody (ABIN967749)

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling Images back to top
Product cited in:

Clark, Hynes: "Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14814-8, 1996

Milasincic, Calera, Farmer, Pilch: "Stimulation of C2C12 myoblast growth by basic fibroblast growth factor and insulin-like growth factor 1 can occur via mitogen-activated protein kinase-dependent and -independent pathways." in: Molecular and cellular biology, Vol. 16, Issue 11, pp. 5964-73, 1996

Sauma, Friedman: "Increased expression of protein kinase C beta activates ERK3." in: The Journal of biological chemistry, Vol. 271, Issue 19, pp. 11422-6, 1996

Cobb, Goldsmith: "How MAP kinases are regulated." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 14843-6, 1995

Images

Product Details anti-MAPK6 Antibody Target Details MAPK6 Application Details Handling References for anti-MAPK6 Antibody (ABIN967749) back to top
Supplier Images
Western Blotting (WB) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (AA 399-543) (ABIN967749) Western blot analysis of ERK3 on a RSV-3T3 lysate. Lane 1: 1:250, lane 2: 1:500, lane...
Immunofluorescence (IF) image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (AA 399-543) (ABIN967749) Immunofluorescent staining of A549 cells. Cells were seeded in a 96 well imaging plat...
 image for anti-MAPK6 antibody (Mitogen-Activated Protein Kinase 6) (AA 399-543) (ABIN967749) anti-Mitogen-Activated Protein Kinase 6 (MAPK6) (AA 399-543) antibody (Image 3)