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anti-Human MAPKAP Kinase 2 antibody for Enzyme Immunoassay

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • AA960234
  • MAPKAP-K2
  • mapkapk2
  • MAPKAPK2
  • MGC78852
  • MK-2
  • MK2
  • Rps6kc1
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
Epitope
pSer272
51
23
21
15
13
13
13
11
10
10
8
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
214
137
108
30
4
2
2
2
1
1
1
Host
Rabbit
191
25
20
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
174
103
89
33
32
28
25
17
10
4
1
1
1
1
Supplier
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Catalog No. ABIN358152
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN117983 EIA IP WB Rabbit AA 310-325 Log in to see Polyclonal 10

Top referenced anti-MAPKAP Kinase 2 antibody for Enzyme Immunoassay

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 86 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Functional Studies (Func) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 18 Antibodies
Intracellular Staining (ICS) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 153 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope pSer272
(51), (23), (21), (15), (13), (13), (13), (11), (10), (10), (8), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(214), (137), (108), (30), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(191), (25), (20)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(174), (103), (89), (33), (32), (28), (25), (17), (10), (4), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN358152) Images
Specificity This antibody detects MAPKAP Kinase-2 (MAPKAPK2) pSer272. Predicted to cross react with Mouse and Rabbit (100 % Antigen Homology).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human MAPKAPK2.
Isotype Ig

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN358152) Images back to top
Antigen
Alternative Name MAPKAP Kinase-2 (MAPKAPK2 Antibody Abstract)
Background This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2
Molecular Weight 45568 Da
Gene ID 9261, 9606
UniProt P49137
Research Area Signaling, Inflammation, Kinases/Phosphatases
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN358152) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details References for anti-MAPKAP Kinase 2 Antibody (ABIN358152) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MAPKAP Kinase 2 Antibody (ABIN358152)

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling Images back to top
Background publications

Lukas, Kroe, Wildeson, Peet, Frego, Davidson, Ingraham, Pargellis, Labadia, Werneburg: "Catalysis and function of the p38 alpha.MK2a signaling complex." in: Biochemistry, Vol. 43, Issue 31, pp. 9950-60, 2004

Underwood, Parris, Federico, Mosyak, Czerwinski, Shane, Taylor, Svenson, Liu, Hsiao, Wolfrom, Maguire, Malakian, Telliez, Lin, Kriz, Seehra, Somers, Stahl: "Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme." in: Structure (London, England : 1993), Vol. 11, Issue 6, pp. 627-36, 2003

Meng, Swenson, Fitzgibbon, Hayakawa, Ter Haar, Behrens, Fulghum, Lippke: "Structure of mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 suggests a bifunctional switch that couples kinase activation with nuclear export." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 37401-5, 2002

Han, Leng, Bian, Mahanivong, Carpenter, Pan, Han, Huang: "Rac1-MKK3-p38-MAPKAPK2 pathway promotes urokinase plasminogen activator mRNA stability in invasive breast cancer cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48379-85, 2002

Werz, Klemm, Samuelsson, Rådmark: "5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5261-6, 2000

Images

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Handling References for anti-MAPKAP Kinase 2 Antibody (ABIN358152) back to top
Supplier Images
 image for anti-MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (pSer272) (ABIN358152) anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pSer272) antibody
 image for anti-MAPKAP Kinase 2 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2) (pSer272) (ABIN358152) anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pSer272) antibody (Image 2)