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anti-Human MAPKAP Kinase 2 antibody for Functional Studies

Recommended MAPKAP Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
  • mapkapk2
  • wu:fb30e10
  • wu:fc56c10
  • wu:fi03a01
  • MGC78852
  • MAPKAPK2
  • AA960234
  • MAPKAP-K2
  • MK-2
  • MK2
  • Rps6kc1
  • mitogen-activated protein kinase-activated protein kinase 2a
  • mitogen-activated protein kinase-activated protein kinase 2
  • MAP kinase-activated protein kinase 2
  • mapkapk2a
  • mapkapk2
  • MAPKAPK2
  • Mapkapk2
Epitope
AA 216-223, pThr222
51
24
20
15
15
15
15
11
9
9
8
6
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rabbit
222
171
137
44
14
8
7
5
5
5
4
4
3
2
2
1
1
Host
Rabbit
194
25
24
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 2 antibody is un-conjugated
8
8
8
6
5
5
4
4
3
3
3
3
3
3
3
3
3
Application
Functional Studies (Func), Western Blotting (WB)
181
97
82
41
39
35
29
17
12
4
4
3
1
Supplier
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Catalog No. ABIN211235
$ 742.50
Plus shipping costs $45.00

Similar anti-MAPKAP Kinase 2 Antibodies

Application / Reactivity Human Mouse (Murine) Hamster Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies
Western Blotting (WB) 161 Antibodies 6 Antibodies 125 Antibodies 9 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 29 Antibodies 4 Antibodies 18 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies 32 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 80 Antibodies 49 Antibodies 2 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) Antibodies
Epitope AA 216-223, pThr222
(51), (24), (20), (15), (15), (15), (15), (11), (9), (9), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rabbit
(222), (171), (137), (44), (14), (8), (7), (5), (5), (5), (4), (4), (3), (2), (2), (1), (1)
Host Rabbit
(194), (25), (24)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 2 antibody is un-conjugated
(8), (8), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Functional Studies (Func), Western Blotting (WB)
(181), (97), (82), (41), (39), (35), (29), (17), (12), (4), (4), (3), (1)
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Product Details anti-MAPKAP Kinase 2 Antibody

Target Details MAPKAP Kinase 2 Application Details Handling Images
Specificity Recognizes human MAPKAP Kinase 2 phosphorylated at Thr222. Species cross-reactivity: hamster, mouse and rabbit.
Purification Protein A purified
Immunogen KLH-conjugated synthetic peptide (C-SHNSLT[ pT]P) corresponding to amino acids 216-223 of phosphorylated human MAPKAP Kinase 2, containing a N-terminal cysteine. The immunizing sequence is identical in mouse, rabbit and hamster.

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details MAPKAP Kinase 2

Product Details anti-MAPKAP Kinase 2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAPKAPK2 / MK2 (MAPKAPK2 Antibody Abstract)
Background Name/Gene ID: MAPKAPK2
Subfamily: MAPKAPK
Family: Protein Kinase

Synonyms: MAPKAPK2, MK-2, MAP KAP Kinase 2, MAPKAP kinase 2, MK2, MAPKAP-K2, MAPKAPK-2
Gene ID 9261
UniProt P49137
Pathways MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades

Application Details

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Handling Images back to top
Application Notes Approved: Func, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL detects 50 ng of recombinant MAPKAP Kinase 2, active, but not 50 ng of recombinant MAPKAP Kinase 2, inactive. May not effectively immunoblot MAPKAP Kinase 2 from cell lines and tissues (lot dependant). Phosphorylated MAPKAP Kinase 2 was detected using 1 μg/mL of antibody. Recombinant, inactive MAPKAP K2, 50 ng, and recombinant, active MAPKAP K2, 50 ng, were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-MAPKAP Kinase 2 (Thr222) (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Non-radioactive Kinase Assay: 0.02 units of p38a/SAPK2a, active phosphorylated 500 ng of MAPKAP Kinase 2, inactive.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 2 Antibody Target Details MAPKAP Kinase 2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.