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anti-Human MAPKAP Kinase 5 antibody for Immunocytochemistry

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAP-K5
  • MAPKAPK5
  • MK-5
  • MK5
  • PRAK
Epitope
Center
23
22
20
14
13
8
7
6
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
147
60
8
2
2
2
Host
Rabbit
100
44
20
Clonality
Polyclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
139
87
41
14
12
9
9
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN2856547
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN1833392 ICC WB Rabbit IgG AA 197-457 Log in to see Polyclonal
0.0009965416 ABIN443623 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.0009965416 ABIN4347179 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-MAPKAP Kinase 5 Antibodies

Application / Reactivity Human
ELISA 71 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 126 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Epitope Center
(23), (22), (20), (14), (13), (8), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(147), (60), (8), (2), (2), (2)
Host Rabbit
(100), (44), (20)
Clonality
Polyclonal
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(139), (87), (41), (14), (12), (9), (9), (2), (2), (1), (1), (1)
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling Images
Cross-Reactivity Mouse (Murine), Chicken, Pig (Porcine), Rat (Rattus), Xenopus tropicalis
Cross-Reactivity (Details) Mouse (96 %), Chicken (95 %), Pig (97 %), Rat (96 %), Xenopus tropicalis (90 %)
Characteristics Rabbit polyclonal antibody to PRAK (mitogen-activated protein kinase-activated protein kinase 5)
PRAK antibody [N3C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.
Isotype IgG

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRAK (MAPKAPK5 Antibody Abstract)
Background The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Cellular Localization: Cytoplasm (Probable) , Nucleus
Molecular Weight 54 kDa
Gene ID 8550
Research Area Cancer
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Images back to top
Format Liquid
Concentration 0.62 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (Center) (ABIN2856547) WB Image Sample(30 μg of whole cell lysate) A:293T B:H1299 7.5% SDS PAGE antibody dil...
Immunofluorescence (IF) image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (Center) (ABIN2856547) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRAK, ...