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MAPKAP Kinase 5 antibody (Internal Region)

Verified MAPKAPK5 Reactivity: Human, Mouse WB, ELISA, IHC, IP Host: Goat Polyclonal unconjugated
Catalog No. ABIN1590011
  • Target See all MAPKAP Kinase 5 (MAPKAPK5) Antibodies
    MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
    Binding Specificity
    • 27
    • 22
    • 7
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 81
    • 49
    • 33
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 86
    • 9
    • 1
    Goat
    Clonality
    • 89
    • 7
    Polyclonal
    Conjugate
    • 40
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MAPKAP Kinase 5 antibody is un-conjugated
    Application
    • 74
    • 38
    • 27
    • 27
    • 15
    • 12
    • 7
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Purpose
    MK5 / MAPKAPK5
    Sequence
    STEALDNVLP SAQ
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product MAPKAPK5 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8 μg/mL.

    Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
    Alternative Name
    Mapkapk5 (MAPKAPK5 Products)
    Synonyms
    MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody
    Background
    MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase
    Gene ID
    8550, 17165, 498183
    NCBI Accession
    NP_003659, NP_620777
    Pathways
    MAPK Signaling
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