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anti-Human MAPKAP Kinase 5 antibody for Immunohistochemistry

Recommended MAPKAP Kinase 5 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
  • MAPKAP-K5
  • MAPKAPK5
  • MK-5
  • MK5
  • PRAK
Reactivity
Human
143
59
7
2
2
2
Host
Mouse
99
41
20
Clonality
Monoclonal
Conjugate
This MAPKAP Kinase 5 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
135
85
38
12
12
9
9
2
2
1
1
Supplier
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Catalog No. ABIN969362
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22356582 ABIN1532150 IHC ELISA WB Rabbit IgG pThr182 Log in to see Polyclonal
0.22356582 ABIN1533990 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22356582 ABIN2464093 IHC ELISA WB Mouse IgG1 truncated Log in to see 7H10B4
0.22356582 ABIN2447987 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.22356582 ABIN931334 IHC ELISA WB Mouse IgG1 Log in to see 7H10B4
0.22356582 ABIN2447989 IHC WB Rabbit Ig Fraction pSer93 Log in to see Polyclonal
0.22356582 ABIN1844770 IHC ELISA WB Mouse IgG1 Log in to see
0.0011431177 ABIN1953034 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN1953906 IHC ELISA WB APC Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN1956347 IHC ELISA WB FITC Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN1955172 IHC ELISA WB Biotin Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN1958679 IHC ELISA WB PE Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN3182699 ELISA IHC WB Rabbit IgG pThr182 Log in to see Polyclonal
0.0011431177 ABIN3186550 ELISA IHC Rabbit IgG Internal Region Log in to see Polyclonal
0.0011431177 ABIN1957551 IHC ELISA WB HRP Rabbit IgG Ser93 Log in to see Polyclonal
0.0011431177 ABIN1452693 IHC ELISA WB Rabbit IgG pThr182, Internal Region Log in to see Polyclonal
0.0011431177 ABIN2884028 IHC ELISA WB Rabbit IgG pThr182 Log in to see Polyclonal
0.0011431177 ABIN2884029 IHC ELISA Rabbit IgG AA 171-220 Log in to see Polyclonal
0.0011431177 ABIN2869255 IHC WB Mouse IgG1 Log in to see
0.0011431177 ABIN2340199 IHC ELISA WB Mouse IgG1 Log in to see 13B640

Top referenced anti-MAPKAP Kinase 5 antibody for Immunohistochemistry

Similar anti-MAPKAP Kinase 5 Antibodies

Application / Reactivity Human
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 122 Antibodies

General

Antigen Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5) Antibodies
Reactivity Human
(143), (59), (7), (2), (2), (2)
Host Mouse
(99), (41), (20)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAPKAP Kinase 5 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(135), (85), (38), (12), (12), (9), (9), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-MAPKAP Kinase 5 Antibody

Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN969362) Images
Purification Ascites
Immunogen Purified recombinant fragment of PRAK expressed in E. coli.
Clone 7H10B4
Isotype IgG1

Target Details MAPKAP Kinase 5

Product Details anti-MAPKAP Kinase 5 Antibody Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN969362) Images back to top
Antigen
Alternative Name PRAK (MAPKAPK5 Antibody Abstract)
Background PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45 % sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
Gene ID 8550
HGNC 8550
Research Area Cancer
Pathways MAPK Signaling

Application Details

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN969362) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details References for anti-MAPKAP Kinase 5 Antibody (ABIN969362) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-MAPKAP Kinase 5 Antibody (ABIN969362)

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling Images back to top
Product cited in:

Paliga, Natale, Watson: "p38 mitogen-activated protein kinase (MAPK) first regulates filamentous actin at the 8-16-cell stage during preimplantation development." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 97, Issue 8, pp. 629-40, 2005

Wijtten, Prak, Lemme, Langhout: "Effect of different dietary ideal protein concentrations on broiler performance." in: British poultry science, Vol. 45, Issue 4, pp. 504-11, 2004

New, Jiang, Han: "Regulation of PRAK subcellular location by p38 MAP kinases." in: Molecular biology of the cell, Vol. 14, Issue 6, pp. 2603-16, 2003

Images

Product Details anti-MAPKAP Kinase 5 Antibody Target Details MAPKAP Kinase 5 Application Details Handling References for anti-MAPKAP Kinase 5 Antibody (ABIN969362) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (ABIN969362) Figure 1: Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A)...
Western Blotting (WB) image for anti-MAPKAP Kinase 5 antibody (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5) (ABIN969362) Figure 2: Western blot analysis using PRAK mouse mAb against truncated PRAK recombina...