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anti-Human MCL-1 antibody for Immunofluorescence

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Epitope
C-Term
34
23
23
16
15
14
13
13
11
10
9
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
331
110
72
10
9
5
5
3
3
2
1
1
1
Host
Rabbit
222
98
20
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
7
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
282
142
114
86
82
52
32
27
25
22
7
5
1
Supplier
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Catalog No. ABIN500252
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN500254 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 1
0.32040194 ABIN500256 EIA IF WB Rabbit IgG Center Log in to see Polyclonal 1
0.32040194 ABIN1531687 IF ELISA Rabbit IgG pSer159 Log in to see Polyclonal
0.32040194 ABIN2453278 IF WB Mouse IgG1 Log in to see 2D6
0.32040194 ABIN2855261 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.32040194 ABIN2448021 IF ELISA Rabbit IgG pSer159 Log in to see Polyclonal
0.32040194 ABIN3210201 IF IHC ELISA WB Mouse IgG1 Log in to see 8C6-8C6D4B1
0.0010840179 ABIN303283 IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal 1
0.0010840179 ABIN2869169 IF IHC WB Mouse IgG1 Log in to see 1
0.0010840179 ABIN1499342 IF IHC (p) WB Mouse IgG2a Log in to see 3B4
0.0010840179 ABIN1499339 IF IHC (p) WB Mouse IgG2a Log in to see 3A12
0.0010840179 ABIN1499340 IF IHC (p) WB Mouse IgG2a Log in to see 9D7
0.0010840179 ABIN1499343 IF IHC (p) WB Mouse IgG1 Log in to see 10C5
0.0010840179 ABIN1499344 IF WB Mouse IgG1 Log in to see 2D6
0.0010840179 ABIN1499341 IF IHC (p) WB Mouse IgG2b Log in to see 9C9
0.0010840179 ABIN1499338 FACS IF IHC (p) WB Mouse IgG1 Log in to see 10F6
0.0010840179 ABIN1499337 FACS IF IHC (p) WB Mouse IgG2a Log in to see 10D10
0.0010840179 ABIN2453283 IF IHC WB Mouse IgG2b Log in to see 9C9
0.0010840179 ABIN2453284 IF IHC WB Mouse IgG2a Log in to see 3B4
0.0010840179 ABIN2453285 IF IHC WB Mouse IgG1 Log in to see 10C5

Top referenced anti-MCL-1 antibody for Immunofluorescence

Similar anti-MCL-1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 20 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 11 Antibodies
Immunofluorescence (IF) 8 Antibodies 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 20 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 16 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 50 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope C-Term
(34), (23), (23), (16), (15), (14), (13), (13), (11), (10), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(331), (110), (72), (10), (9), (5), (5), (3), (3), (2), (1), (1), (1)
Host Rabbit
(222), (98), (20), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(282), (142), (114), (86), (82), (52), (32), (27), (25), (22), (7), (5), (1)
Pubmed 1 reference available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN500252) Images
Specificity This antibody detects MCL1 at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Ion exchange chromatography
Immunogen Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human Mcl-1.
Isotype IgG

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN500252) Images back to top
Antigen
Alternative Name MCL1 (MCL1 Antibody Abstract)
Background Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival (reviewed in 1). While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak (2,3). Mcl-1 was originally identified in differentiating myeloid cells (4), but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis (5) and for the development and maintenance of B and T lymphocytes in animals (3). Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death (6).Synonyms: BCL2L3, Bcl-2-like protein 3, Bcl-2-related protein, EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, mcl1/EAT, r004
Gene ID 4170
NCBI Accession NP_068779
UniProt Q07820
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN500252) Images back to top
Application Notes ELISA: Western blot: 1 to 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN500252) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-MCL-1 Antibody (ABIN500252)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Background publications

Edwards, Derouet, Howse, Moots: "Regulation of neutrophil apoptosis by Mcl-1." in: Biochemical Society transactions, Vol. 32, Issue Pt3, pp. 489-92, 2004

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN500252) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term) (ABIN500252) Immunocytochemistry staining of Raji cells using AP30547PU-N at 2 μg/ml.
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term) (ABIN500252) Western blot analysis of Mcl-1 in Raji cell lysates with this product at (A) 1 and (B...