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anti-Human MCL-1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended MCL-1 Antibody (supplied by: Log in to see )

Antigen
Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Epitope
AA 121-139
35
23
23
16
15
13
13
13
11
10
9
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
331
111
73
10
9
5
5
3
3
2
1
1
1
Host
Rabbit
222
98
20
2
Clonality
Polyclonal
Conjugate
This MCL-1 antibody is un-conjugated
12
10
10
7
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
282
142
114
86
83
52
32
26
24
22
7
6
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Catalog No. ABIN967399
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Top referenced anti-MCL-1 antibody for Immunohistochemistry (Formalin-fixed Sections)

Similar anti-MCL-1 Antibodies

Application / Reactivity Human
ELISA 135 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunofluorescence (IF) 83 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 114 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 276 Antibodies

General

Antigen Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) Antibodies
Epitope AA 121-139
(35), (23), (23), (16), (15), (13), (13), (13), (11), (10), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(331), (111), (73), (10), (9), (5), (5), (3), (3), (2), (1), (1), (1)
Host Rabbit
(222), (98), (20), (2)
Clonality
Polyclonal
Conjugate This MCL-1 antibody is un-conjugated
(12), (10), (10), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(282), (142), (114), (86), (83), (52), (32), (26), (24), (22), (7), (6)
Pubmed 6 references available
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Product Details anti-MCL-1 Antibody

Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN967399) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. This product contains thimerosal, an organic mercury compound. Mercury and mercury compounds are chemicals known to the State of California to cause birth defects or other reproductive harm. Foreseeable use of this product does not pose a known reproductive toxicity threat.
4. Please refer to us for technical protocols.
Purification Serum
Immunogen Human Mcl-1 aa. 121-139 Peptide
Isotype Ig Fraction

Target Details MCL-1

Product Details anti-MCL-1 Antibody Application Details Handling References for anti-MCL-1 Antibody (ABIN967399) Images back to top
Antigen
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background Mcl-1 (for myeloid cell leukemia-1) is a member of the Bcl-2 family that was identified by screening of of cDNA libraries derived from differentiating human myeloid leukemia cells. Bcl-2 family members are involved in apoptosis and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members including Bcl-2 act as inhibitors of apoptosis, whereas others such as Bax promote cell death. It is thought that protein-protein interactions between Bcl-2 family members play an important role in their function. For example, Bax can homodimerize as well as heterodimerize with Bcl-2. When Bax is present in excess, it can counteract the ability of Bcl-2 to inhibit cell death. Although its function remains to be fully elucidated, Mcl-1 may, like Bcl-2, be a blocker of cell death. For example, transfection of Mcl-1 into Chinese hamster ovary cells partially blocked Myc-induced apoptosis, and Mcl-1 blocked Bax mediated cell death in a yeast two-hybrid system. The two-hybrid system results suggest that Mcl-1/Bax interations may have functional homology to Bcl-2/Bax interations in blocking Bax-mediated cell death. This polyclonal antibody is not recommended for the identification of mouse Mcl-1 and has been reported not to cross-react with human Bcl-2.
Molecular Weight 39-42 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling

Application Details

Product Details anti-MCL-1 Antibody Target Details MCL-1 Handling References for anti-MCL-1 Antibody (ABIN967399) Images back to top
Comment

Related Products: ABIN967390

Restrictions For Research Use only

Handling

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details References for anti-MCL-1 Antibody (ABIN967399) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide and ≤0.03 % thimerosal
Preservative Sodium azide, Thimerosal (Merthiolate)
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-MCL-1 Antibody (ABIN967399)

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling Images back to top
Product cited in:

Ohta, Iwai, Kasahara, Taniguchi, Krajewski, Reed, Miyawaki: "Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues." in: International immunology, Vol. 7, Issue 11, pp. 1817-25, 1996

Reynolds, Yang, Qian, Jenkinson, Zhou, Eastman, Craig: "Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells." in: Cancer research, Vol. 54, Issue 24, pp. 6348-52, 1995

Krajewski, Bodrug, Gascoyne, Berean, Krajewska, Reed: "Immunohistochemical analysis of Mcl-1 and Bcl-2 proteins in normal and neoplastic lymph nodes." in: The American journal of pathology, Vol. 145, Issue 3, pp. 515-25, 1994

Sato, Hanada, Bodrug, Irie, Iwama, Boise, Thompson, Golemis, Fong, Wang: "Interactions among members of the Bcl-2 protein family analyzed with a yeast two-hybrid system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 20, pp. 9238-42, 1994

Kozopas, Yang, Buchan, Zhou, Craig: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993

Background publications

Krajewski, Bodrug, Krajewska, Shabaik, Gascoyne, Berean, Reed et al.: "Immunohistochemical analysis of Mcl-1 protein in human tissues. Differential regulation of Mcl-1 and Bcl-2 protein production suggests a unique role for Mcl-1 in control of programmed cell death in ..." in: The American journal of pathology, Vol. 146, Issue 6, pp. 1309-19, 1995

Images

Product Details anti-MCL-1 Antibody Target Details MCL-1 Application Details Handling References for anti-MCL-1 Antibody (ABIN967399) back to top
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 121-139) (ABIN967399) Western blot analysis for Mcl-1. Left figure: In vitro translated human Mcl-1 (lane 1...
 image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 121-139) (ABIN967399) A SK-N-SH cell lysate (Human neuroblastoma, ATCC HTB-11) was probed with the polyclon...
 image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (AA 121-139) (ABIN967399) anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (AA 121-139) antibody (Image 3)